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pcDNA3-HA-14-3-3 beta

Plasmid

#13270

Depositor

Michael Yaffe

Insert

14-3-3 beta (YWHAB Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLOX-CWBmi1

Plasmid

#12240

Depositor

Didier Trono

Publication

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

Bmi1 (Bmi1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter upstream of mBmi-1. LoxP in 3' …

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Publication

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Publication

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Publication

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Publication

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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Cbh_v5 AAV-CBE N-terminal

Plasmid

#137175

Purpose

AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE N-terminal; U6-protospacer

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

Plasmid

#135391

Purpose

Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

Depositor

Fan Wang

Insert

TeLC-P2A-EYFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Expression

Bacterial

Promoter

native phage promoters

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Halotag-FRB-EX-nes_pLX304

Plasmid

#120913

Purpose

Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

HA-Halotag-FRB-EX-NES

Use

Lentiviral

Expression

Mammalian

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJMP1039

Plasmid

#119239

Purpose

expresses Tn7 transposase

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

tnsABCD

Expression

Bacterial

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Publication

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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