4,143 results

1. pcDNA3-Camui-CR

   Plasmid

   #40256

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Camuiα-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

   Plasmid

   #64222

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV-T7-hOsa2

   Plasmid

   #17987

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa2 (ARID1B Human)
   

   Use

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEYFP SUMO-1

   Plasmid

   #13380

   Depositor

   Mary Dasso

   Article

   Ayaydin et al Mol Biol Cell. 2004 Dec . 15(12):5208-18

   Insert

   SUMO-1 (SUMO1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMSCVpuro-Flag-cMyc T58A

   Plasmid

   #20076

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   cMyc (MYC Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T58A

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-miR21

   Plasmid

   #21114

   Depositor

   Joshua Mendell

   Article

   Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   miR-21 (MIR21 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn-Chronos-GFP

   Plasmid

   #59170

   Purpose

   AAV-mediated expression of Chronos-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMax-E2F1

   Plasmid

   #16007

   Depositor

   Olimpia Meucci

   Article

   Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBabe N-Ras 61K

   Plasmid

   #12543

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   NRas 61K (NRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   61K constitutively activated.

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pY002 (pFnCpf1_min)

    Plasmid

    #69975

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes nd artificial promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTDH3-dCas9

    Plasmid

    #46920

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. B23(NPM)

    Plasmid

    #34553

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    B23/nucleophosmin (NPM1 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tdTomato-Lifeact-7

    Plasmid

    #54528

    Purpose

    Localization: Actin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Type

    Empty backbone

    Use

    Tags

    tdTomato-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-HA-BRCC3

    Plasmid

    #22540

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    BRCC3 (BRCC3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pN1 Lck-GCaMP5G

    Plasmid

    #34924

    Purpose

    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

    Depositor

    Baljit Khakh

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    Lck-GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-eNOS-GFP

    Plasmid

    #22444

    Depositor

    William Sessa

    Article

    Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

    Insert

    WT eNOS-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEZY3

    Plasmid

    #18672

    Purpose

    pcDNA3 converted Gateway Destination vector for mammalian expression

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGKneoF2L2DTA

    Plasmid

    #13445

    Depositor

    Philippe Soriano

    Article

    Hoch et al Development. 2006 Feb . 133(4):663-73.

    Insert

    PGKneoF2L2DTA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TtRMPVIR

    Plasmid

    #27995

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TtRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge