19,635 results

1. LZ10 PBREBAC-H2BHalo

   Plasmid

   #91564

   Purpose

   piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

   Depositor

   James Zhe Liu

   Article

   Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

   Insert

   histone H2B-HaloTag
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EB3-mScarlet-I

   Plasmid

   #98826

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-FOXM1c

   Plasmid

   #68810

   Purpose

   Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

   Depositor

   Adam Karpf

   Article

   Barger et al Oncotarget. 2015 Jul 16.

   Insert

   FOXM1c (FOXM1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen_taq

   Plasmid

   #153315

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   Drew Endy

   Insert

   Taq polymerase
   

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP_sfCherry2(11)_H2B

   Plasmid

   #82605

   Purpose

   Expresses sfCherry2(11) tagged H2B in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_H2B
   

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPX4

   Plasmid

   #38797

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GPX4 (GPX4 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   SeCys73 -> Gly

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. psfaRb-Tn5

   Plasmid

   #183638

   Purpose

   Expresses anti-rabbit nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti rabbit secondary nanobody fused to Tn5
   

   Expression

   Bacterial

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Tags

   6X His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pet28a_Human_H3.1

    Plasmid

    #42631

    Depositor

    Joe Landry

    Insert

    Human H3.1 (H3C1 Human)
    

    Tags

    Untagged

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pFRT_TO_DESTFLAGHA

    Plasmid

    #26361

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Type

    Empty backbone

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. SypHer

    Plasmid

    #48250

    Purpose

    pH sensor

    Depositor

    Nicolas Demaurex

    Article

    Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

    Insert

    SypHer
    

    Expression

    Mammalian

    Mutation

    C199S

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAM1579

    Plasmid

    #40240

    Depositor

    Susan Golden

    Article

    Andersson et al Methods Enzymol. 2000;305:527-42.

    Insert

    Km Resistance, neutral site II integration platform for Synechococcus
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. YFP-Parkin

    Plasmid

    #23955

    Purpose

    Mammalian expression of human Park2 fused to EYFP

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Park2 (PRKN Human)
    

    Tags

    eYFP

    Expression

    Mammalian

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW12.lentiguide.GFP

    Plasmid

    #104374

    Purpose

    GFP vector for cloning in guides

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTTH+H1.4

    Plasmid

    #157796

    Purpose

    Expression of human linker histone H1.4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4 (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLsinPGK_IRFP720_WPRE

    Plasmid

    #91785

    Purpose

    Constitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    iRFP720
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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