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Showing: 7721 - 7760 of 10232 results

  17. pPOLY_new

    Plasmid
    #179276
    Purpose
    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    Use
    Synthetic Biology
    Tags
    6xHIS and Strep-Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179276-plasmid-map-sequence-id-358730

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  25. pRSF-G1pCNPRS

    Plasmid
    #174719
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174719-plasmid-map-sequence-id-346622

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  26. NmMetQ C20A

    Plasmid
    #172112
    Purpose
    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Use
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine on MetQ and Cystein…
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172112-plasmid-map-sequence-id-344822

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  27. pPodA11

    Plasmid
    #162843
    Purpose
    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance
    Depositor
    Dianne Newman
    Article
    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11
    Insert
    Protein Designed Pyocyanin demethylase
    Use
    Tags
    TEV-cleavable 6x-His tag
    Expression
    Bacterial
    Mutation
    A53N, I73T, A87V, M99V, A129T from WT PodA
    Promoter
    T7
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162843-plasmid-map-sequence-id-343491

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  28. pENTR-D-kcnj13

    Plasmid
    #173143
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173143-plasmid-map-sequence-id-343318

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  29. pENTR-D-kcnj1b

    Plasmid
    #173126
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj1b (kcnj1b Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173126-plasmid-map-sequence-id-342841

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  30. pDONR221-kcnj8

    Plasmid
    #173134
    Purpose
    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
    Depositor
    GuangJun Zhang
    Article
    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.
    Insert
    kcnj8 (kcnj8, si:dkey-183c2.2 Zebrafish)
    Use
    Pcr cloning vector
    Tags
    Expression
    Mutation
    Promoter
    No promoter at 5 end; T7 promoter at 3 end.
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173134-plasmid-map-sequence-id-343157

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  33. Ash1-5p

    Plasmid
    #169712
    Purpose
    Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
    Depositor
    Tanja Mittag
    Article
    Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-480…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169712-plasmid-map-sequence-id-334784

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  34. pET28a-SdrG-FLN-ELP-His-ybbr

    Plasmid
    #168047
    Purpose
    Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
    Depositor
    Michael Nash
    Article
    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, ddFLN4, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168047-plasmid-map-sequence-id-334769

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  35. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168476-plasmid-map-sequence-id-332734

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  36. pET11a-Z-N-DiB-RM

    Plasmid
    #168475
    Purpose
    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168475-plasmid-map-sequence-id-332732

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Showing: 7721 - 7760 of 10232 results