165,144 results

1. pPUR-mU6-sgRNA-2XPP7

   Plasmid

   #194504

   Purpose

   Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR-attL5-CRY2-mCh-attL2

   Plasmid

   #160438

   Purpose

   Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

   Depositor

   Nicholas Tolwinski

   Article

   Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCZGY2254

   Plasmid

   #101247

   Purpose

   GFP subunits 1-10, (only shows active fluorescence when binds to GFP11)

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   gfp1-10
   

   Use

   Gateway

   Tags

   GFP1-10

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-HLA-E-COMP5AP-AviTag-9xHis

   Plasmid

   #157396

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   HLA-E (HLA-E Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas12j3 VPR

   Plasmid

   #188517

   Purpose

   Expresses FLAG tagged dCas12j3 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRK246

   Plasmid

   #173741

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   wrt-2 promoter
   

   Use

   Other

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD373

   Plasmid

   #137021

   Purpose

   SapTrap GFP-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized GFP
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS714

    Plasmid

    #188886

    Purpose

    MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-SMARCA4

    Plasmid

    #65391

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SMARCA4 (SMARCA4 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-SNAP-PI(3,4)P2 Probe

    Plasmid

    #211511

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    TAPP1 cPHx3
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPHK1 gRNA (BRDN0001147534)

    Plasmid

    #76814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPHK2 gRNA (BRDN0001146882)

    Plasmid

    #77096

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS1NB m Rosella I

    Plasmid

    #71247

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Tags

    yeast mitochondrial targeting sequence of citrate…

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. Str-2-TagBFP-SL2-Pre-SPHERE-SF-iGluSnFR

    Plasmid

    #187099

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-SF-iGluSnFR
    

    Tags

    TagBFP-SL2

    Expression

    Worm

    Promoter

    Str-2

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. M87

    Plasmid

    #242633

    Purpose

    MYC expression

    Depositor

    Alain Nepveu

    Article

    Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    EGFP-MYC (MYC Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. DAP12-SmBiT BiBiT Ready Vector

    Plasmid

    #238799

    Purpose

    Express DAP12-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    DAP12 (TYROBP Human)
    

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. IL4Ra-HaloTag BiBRET Vector

    Plasmid

    #238676

    Purpose

    Express IL4Ra-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IL4Ra (IL4R Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pLenti-eIF6-3XFlag

    Plasmid

    #242188

    Purpose

    Express eIF6-3xFlag in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    eIF6 (EIF6 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Promoter

    CMV

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AiP12320 - pAAV-AiE0475m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2320)

    Plasmid

    #229885

    Purpose

    AiE0475m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGP-AAV-syn-FLEX-Positron2-ST-WPRE

    Plasmid

    #239080

    Purpose

    AAV-mediated expression of positive-going voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Eric R. et al Janelia Figshare 2023

    Insert

    Positron2-ST
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    R78K N81D D92N W178F

    Promoter

    hSynapsin1

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCDH-CB-Cdkn1a

    Plasmid

    #228914

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Expression

    Mammalian

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDNA5/FRT_CMV_NES-Kinprola_PKA-mEGFP

    Plasmid

    #233353

    Purpose

    CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mEGFP
    

    Tags

    NES and mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Str-KDEL_SBP-mCherry-CCR1

    Plasmid

    #222311

    Purpose

    Synchronize the trafficking of CCR1 from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge