We narrowed to 13,646 results for: NTS

1. pMOD_A1610

   Plasmid

   #91038

   Purpose

   Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-TaCas9_D10A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   ZmUbi

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGM65893

   Plasmid

   #153216

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJKW1813

   Plasmid

   #174003

   Purpose

   Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

   Depositor

   Jing-Ke Weng

   Article

   Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

   Inserts

   PpCYP90G4
   ATR1 (ATR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mVenus-CENPB-N-22

   Plasmid

   #56325

   Purpose

   Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   CENPB (CENPB Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   aa 1-169 of CENPB (DNA Binding Domain)

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pJBL704

   Plasmid

   #140374

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-4BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWB424

   Plasmid

   #74818

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB429

   Plasmid

   #74823

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGoldyTALEN-TUBA1B-TAL-2

   Plasmid

   #62854

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCAGG

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGoldyTALEN-TUBA1B-TAL-1

   Plasmid

   #62853

   Purpose

   TALEN mediated gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   TALEN array
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCAGG

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Apr 9. ():.

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pJBL705

    Plasmid

    #140375

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-5BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTaCas9_6

    Plasmid

    #91169

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWB407

    Plasmid

    #74801

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFAB822

    Plasmid

    #47856

    Purpose

    BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    BBa_B1006 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO11

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFAB803

    Plasmid

    #47847

    Purpose

    BIOFAB reporter plasmid for measuring T7 early termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    T7 early terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO2

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. prNFL-Q333P-EGFP

    Plasmid

    #132602

    Purpose

    Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E Q333P mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    Nefl (Nefl Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    998A>C, 999G>C; Gln333Pro

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KHBD00433

    Plasmid

    #34538

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9745 (D1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197965

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-nCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KHBD00606

    Plasmid

    #39684

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG15715 (CG15715 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

21. phNFL-E396K

    Plasmid

    #132595

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1186G>A; Glu396Lys

    Promoter

    CMV

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUPD2 AtUBQ10 (GB2478)

    Plasmid

    #160594

    Purpose

    UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtUBQ10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOPO636

    Plasmid

    #195299

    Purpose

    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABCQ operon of McCAST (Tn7575)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG inducible lac promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB452

    Plasmid

    #74834

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMOD_A1810

    Plasmid

    #91040

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

26. FusionRed-PlastinC-10

    Plasmid

    #56130

    Purpose

    Localization: Cytoskeleton, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Plastin (PLS1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-BirA-eCPX-AP(K10)

    Plasmid

    #121908

    Purpose

    Expresses BirA and eCPX-AP(K10)

    Depositor

    Per Svenningsen

    Article

    Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

    Inserts

    BirA (birA Synthetic)
    enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 10 in the AP to alanine

    Promoter

    T7 and pBAD promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmalc4x_I499F_TEV

    Plasmid

    #47703

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with I499F mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed I499F

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge