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YFP-Rpp38-C1

Plasmid

#134548

Purpose

Expresses YFP-Rpp38 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp38 (RPP38 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-His

Plasmid

#67866

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP3 (KIAA0391 Human)

Tags

His

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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ama1CD4d3+4-bio

Plasmid

#36145

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pRT006.3 L1 dual-luciferase AI retrotransposition reporter

Plasmid

#213031

Purpose

Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

Depositor

Donna Romero, Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker

Use

Luciferase

Tags

Firefly luciferase antisense intron (3' UTR)

Expression

Mammalian

Promoter

pTRE bidirectional tet-on

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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LifeAct-mGarnet

Plasmid

#104307

Purpose

codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

LifeAct-mGarnet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔN

Plasmid

#35184

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-264

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
PL-452 C-mDsRed

Plasmid

#19179

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

mDsRed

Use

Cre/Lox

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
HOXB4 (human) FLAG MSCV

Plasmid

#8528

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Missing N-terminal 14 amino acids; improved Ribos…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GFP-Flag-UTR

Plasmid

#183235

Purpose

To express FLAG tagged eGFP

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Article

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Insert

Insulator BRE-MLP:H2B-Turqoise

Expression

Mammalian

Promoter

BRE-MLP

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAN1717

Plasmid

#74696

Purpose

RPU standard plasmid

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Insulated J23101-YFP

Expression

Bacterial

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTH-TelR15-mVenus

Plasmid

#49639

Purpose

TALEColor-mVenus-Telomere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTH-TelL20-mVenus

Plasmid

#49636

Purpose

TALEColor-mVenus-Telomere

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Type

Empty backbone

Use

TALEN

Tags

TelL20

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA TrkC

Plasmid

#24089

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkC (Ntrk3 Rat)

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-9

Plasmid

#208787

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 9 (CASP9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pM2s2TsR

Plasmid

#137923

Purpose

E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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TagBFP_A1aY1

Plasmid

#158752

Purpose

Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagBFP-T_A1aY1

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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