We narrowed to 108 results for: IMD2

1. pFLAG-GATA2-D377-415

   Plasmid

   #1421

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 377 to 415

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pFLAG-GATA2-D415-475

   Plasmid

   #1422

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 414 to 475

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pBabeHygro-GATA2-Ala(381-385)

   Plasmid

   #1416

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   alanine substitutions at amino acids 381-385

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pBabeHygro-GATA2-D415-475

   Plasmid

   #1415

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 415 to 475

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. PX458-Lck

   Plasmid

   #159430

   Purpose

   CRISPR knockout of Lck

   Depositor

   Arthur Salomon

   Article

   Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

   Insert

   sgRNA targeting Lck exon 2 (LCK Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LIC-Z-Y-L

   Plasmid

   #153546

   Purpose

   this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   with 6 Y in 3 ITAMs mutated to L

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac(His10-Lckg2a,Y394F,Y505F)

   Plasmid

   #177894

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Use

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A,Y394F,Y505F

   Promoter

   Polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMXs-GATA2

   Plasmid

   #131602

   Purpose

   expression of human GATA2 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   GATA2 (GATA2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEM-human TCR zeta/2470

   Plasmid

   #11507

   Depositor

   Allan Weissman

   Article

   Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

   Insert

   TCR zeta (CD247 Human)
   

   Use

   General cloning

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

10. LIC-Z

    Plasmid

    #153543

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHH0103_LCK-1/1

    Plasmid

    #91332

    Purpose

    Protein expression and purification of human SH3 domain construct LCK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    LCK-1/1 (LCK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLAG-GATA2-Ala(381-385)

    Plasmid

    #1423

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

    Insert

    GATA-2 (GATA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alanine substitutions at amino acids 381-385

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pFLAG-GATA2-Ala(388-394)

    Plasmid

    #1424

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

    Insert

    GATA-2 (GATA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alanine substitutions at amino acids 388-393; M39…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. LIC-Z-delCry2

    Plasmid

    #153545

    Purpose

    this is the non-clustering version of LIC-Z tagged with mcherry

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    with Cry2 sequence deleted from LIC-Z

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBacHT (His10-4xFRB-SNAP-Lck G2A)

    Plasmid

    #177887

    Purpose

    Baculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Use

    Tags

    10xHis-TEV-4xFRB-SNAP tag

    Expression

    Insect

    Mutation

    G2A

    Promoter

    Polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IFNGR2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176939

    Purpose

    Mammalian expression vector encoding IFNGR2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    IFNGR2 (IFNGR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFUW-tetO-GATA2

    Plasmid

    #125028

    Purpose

    doxycycline-inducible expression of human GATA2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    GATA binding protein 2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His10-CD3z-SNAPf-ybbR (XSB507)

    Plasmid

    #78508

    Purpose

    Construct for expressing CD3z-SNAPf-ybbR in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD3z (Cytoplasmic domain) (CD247 Human)
    

    Use

    Tags

    His10 and SNAPf-ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSIN4-EF1a-GATA2-IRES-Puro

    Plasmid

    #61063

    Purpose

    lentiviral vector for GATA2

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LIC-Z-YFP

    Plasmid

    #153544

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    Venus YFP

    Expression

    Mammalian

    Mutation

    with mCherry in LIC-Z replaced by YFP

    Promoter

    CMV

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMTgR2

    Plasmid

    #107286

    Purpose

    Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

    Depositor

    Bohdan Schneider

    Article

    Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

    Insert

    Interferon gamma receptor 2 (IFNGR2 Human)
    

    Use

    Tags

    CATCATCACCATCACCATGA

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF6-Lck-TurboID

    Plasmid

    #159433

    Purpose

    expresses Lck-TurboID in mammalian cells

    Depositor

    Arthur Salomon

    Article

    Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

    Inserts

    Lck (LCK Human)
    TurboID (BirA mutant)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    EF-1a

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. DNA-PKcs PQR>ala

    Plasmid

    #83319

    Purpose

    human DNA-PKcs PQR>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 phosphorylation sites in the PQR cluster substi…

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DNA-PKcs ABCDE>A

    Plasmid

    #83318

    Purpose

    human DNA-PKcs/ABCDE>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    6 phosphorylation sites in the ABCDE cluster subs…

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUW-tetO-3xFLAG-GATA2

    Plasmid

    #125600

    Purpose

    doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    GATA binding protein 2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CmCitrine Lck w/ NosUTRs

    Plasmid

    #66785

    Purpose

    expression of fluorescent membranes in sea urchin primordial germ cells

    Depositor

    Amro Hamdoun

    Article

    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

    Insert

    Lck (LCK Human)
    

    Use

    Sea urchin, zebrafish, xenopus

    Tags

    3' UTR from sea urchin Nanos, 5' UTR fr…

    Expression

    Mutation

    Tandem repeat of LCK the N-terminal membrane targ…

    Promoter

    T7

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

29. DNA-PKcs T3950D

    Plasmid

    #83320

    Purpose

    DNA-PKcs T3950A

    Depositor

    Katheryn Meek

    Article

    Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Activation loop phosphorylation site 3950 substit…

    Promoter

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF2998

    Plasmid

    #144474

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF2997

    Plasmid

    #144473

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pD649-HAsp-FCGR3A-COMP5AP-AviTag-9xHis

    Plasmid

    #157167

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FCGR3A (FCGR3A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTR-452 tNGFR-P2A-Lck

    Plasmid

    #186080

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    LCK (LCK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LCK gRNA (BRDN0001145572)

    Plasmid

    #76411

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LCK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LCK (LCK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LCK gRNA (BRDN0001145503)

    Plasmid

    #76412

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LCK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LCK (LCK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LCK gRNA (BRDN0001145758)

    Plasmid

    #76413

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LCK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LCK (LCK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    flag-halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K3753R

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RMCE wild type DNA-PKcs

    Plasmid

    #233911

    Purpose

    Low copy number provides expression of human DNA-PKcs

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    dna-pkcs cDNA (PRKDC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DNA-CARzeta-GFP

    Plasmid

    #89344

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta (CD247, CD86 Human)
    

    Use

    Lentiviral

    Tags

    SNAPf tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge