Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,219 results for: cas12

Clear keywords
Showing: 41 - 80 of 1219 results

  1. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

    Enlarge

  10. pJV53-Cas12a

    Plasmid
    #158706
    Purpose
    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158706-plasmid-map-sequence-id-320865

    Enlarge

  11. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

    Enlarge

  13. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

    Enlarge

  35. pNHEJ-Cas12a

    Plasmid
    #158713
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575)
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158713-plasmid-map-sequence-id-320845

    Enlarge

  37. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Article
    Lawrenson et al bioRxiv 2022.04.28.489853
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available Since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

    Enlarge

  39. rnLbCas12a-PE2

    Plasmid
    #213746
    Purpose
    For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    rnLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, H759A, R1138A in LbCas12a
    Promoter
    CMV
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213746-plasmid-map-sequence-id-422194

    Enlarge

Showing: 41 - 80 of 1219 results