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Showing: 51 - 100 of 140 results

  10. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid
    #189889
    Purpose
    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AKT1 (AKT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    189889-plasmid-map-sequence-id-374470

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  11. p663-UBC-miniTurbo-V5-AKT1_IDG-K

    Plasmid
    #189890
    Purpose
    Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AKT1 (AKT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189890-plasmid-map-sequence-id-374279

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  12. pLVX-IRES-tdTomato-FlagAkt1W80A

    Plasmid
    #64833
    Purpose
    cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug
    Depositor
    Eva Gonzalez
    Article
    Kajno et al Biochem J. 2015 Apr 9.
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Point mutation from Trp to Ala at position 80
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. R777-E002 Hs.AKT1-nostop

    Plasmid
    #70286
    Purpose
    Gateway ORF clone of human AKT1 [NM_005163.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT1 (AKT1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70286-plasmid-map-sequence-id-125735

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  29. pHRIG-AktDN

    Plasmid
    #53597
    Purpose
    lentiviral expression of dominant negative mouse Akt1 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Dominant negative (K179M)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA3 HA PKB AAA

    Plasmid
    #16001
    Depositor
    Morris Birnbaum
    Insert
    Akt1 (AKT1 Bovine)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K179A, T308A, S473A
    Promoter
    Available since
    Oct. 26, 2007
    Availability
    Academic Institutions and Nonprofits only

  32. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Use
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  34. pBSFI-myr-akt-S473A

    Plasmid
    #49190
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector
    Depositor
    Peter Vogt
    Article
    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    S473A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pBSFI-myr-akt-T308A

    Plasmid
    #49189
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector
    Depositor
    Peter Vogt
    Article
    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    T308A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Lawrence Kane, Arthur Weiss
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Promoter
    Available since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. HA-PKB-pcDNA3-K179A

    Plasmid
    #24652
    Depositor
    Jim Woodgett
    Insert
    PKB (AKT1 )
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K179A
    Promoter
    Available since
    June 1, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 51 - 100 of 140 results