3,641 results
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Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-syt1-GCaMP6f
Plasmid#190896PurposeGCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.DepositorInsertsyt1-GCaMP6f
ExpressionInsectAvailable SinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
Plasmid#217510PurposeKD Recombinase dependent conditional smGdP-10XV5 cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
TagssmGdP-10XV5ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7
Plasmid#172067PurposeProtein trap plasmid for inserting GFP11x7 into a phase 1 coding intronDepositorInsertGFP11x7
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
w+attB
Plasmid#30326DepositorInsertphiC31 attB, hsp70::w
UseDrosophila transgenesisAvailable SinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQUAST
Plasmid#24349DepositorInsert5xQUAS-Pmin promoter
ExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo
Plasmid#73164Purposemulti-cistronic Vector for expression for Fly_FUCCI probes in insect cellsDepositorTagsmRFP1, GFPExpressionInsectMutationCyclin B Fragment aa 1-266 & E2F1 Fragment aa…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
WT (DVD)
Plasmid#184316PurposeTo study dynamics of G-actin using NMR spectroscopy.DepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmPABPC1_J
Plasmid#146647PurposeBacterial Expression of DmPABPC1DepositorAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
SCP2-CMVenh-luc
Plasmid#22230DepositorInsertSCP2 (super core promoter 2)-CMVenh luciferase
UseLuciferaseExpressionMammalianAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
ExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMesh1*
Plasmid#175591PurposeLac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
TagsFused with a flexible GS linker to ceruleanExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC12-10XUAS-IVS-myr::GFP
Plasmid#26222DepositorInsertmyr::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
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PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
ExpressionInsectAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDmAct5C::Cas9-2A-Neo
Plasmid#176679PurposeExpression of Drosophila codon optimized spCas9 under Drosophila Act5C promoterDepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterD. melanogaster Act5CAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab5
Plasmid#31733DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRPromoterU6:1, U6:3, and dU6-1; dU6-3Available SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-mCherry
Plasmid#72894PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorInsertdmBACCS2-IRES-dOrai-IRES-mCherry
TagsIRES-mCherryExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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SCP1-CMVenh-luc
Plasmid#22216DepositorInsertSCP1 (super core promoter 1)-CMVenh luciferase
UseLuciferaseExpressionMammalianAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-dCLK
Plasmid#33153DepositorAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-IMD-V5-His
Plasmid#63748PurposeExpresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertimmune deficiency (imd Fly)
TagsV5-6XHisExpressionInsectMutation*see comment belowPromoterpMTAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-yki:V5-S111A, S168A, S250A
Plasmid#85620PurposeExpresses V5 tagged triple mutant yki in Drosophila.DepositorInsertyki-V5-S111A,S168,S250A
TagsV5ExpressionInsectAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWalium-ChtVis-Tomato
Plasmid#67756PurposeExpresses a fluorescent chitin reporter under Gal4 control in Drosophila.DepositorInsertdyl - chitin binding domain - tdTomato - dyl
Tagstd-tomatoExpressionInsectAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP156 pUAST EGFP:Gr21a
Plasmid#59524PurposeN-terminal fusion of EGFP to Drosophila melanogaster Gr21aDepositorAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectAvailable SinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
K560GFP
Plasmid#129761PurposeExpresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assaysDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
ExpressionInsectPromoterDrosophila metallothionein promoterAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-dCry-V5His
Plasmid#136241PurposeProtein expression of D. melanogaster (Dm) cytochrome with V5 and His tagsDepositorInsertDmCry
TagsHis and V5ExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
Plasmid#217515PurposeEncodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD RecombinaseDepositorInsert13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
ExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-ebony
Plasmid#83380PurposeDrosophila gRNA expression plasmid targets ebonyDepositorAvailable SinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP185 pUAST EGFP:Or47b
Plasmid#59525PurposeN-terminal fusion of GFP to Drosophila melanogaster Or47bDepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP132 pUAST EGFP:Or43a
Plasmid#59523PurposeN-terminal fusion of EGFP to Drosophila melanogaster Or43aDepositorAvailable SinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-GFP-Msp300KASH
Plasmid#170806PurposeExpresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesisDepositorInsertGFP-Msp300KASH
TagsGFPExpressionInsectPromoter5xUAS + hsp70 minimal promoterAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Renilla luciferase-Pol III
Plasmid#37380DepositorInsertPolIII-Renilla control reporter (RpIII128 Fly)
UseLuciferaseExpressionMammalianPromoterRNA PolIII 128 subunit (RpIII128)Available SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Human, Fly, Aequorea victoria)
TagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …Available SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
p026 pCS2-EnR
Plasmid#11028DepositorInsertengrailed (en Fly)
UseXenopus, mammalian, avian, and zebrafishMutationEngrailed repressor domain (bases encoding amino …Available SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)
Plasmid#206434PurposeVasa 463-661 expression in Schneider cells for imagingDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)
Plasmid#206461PurposeOskar 139-240 expression in Schneider cells for imagingDepositorAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
ExpressionInsectAvailable SinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
KHBD00727
Plasmid#39748DepositorAvailable SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
nSYB-GAL80-DD
Plasmid#124778PurposeExpresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoterDepositorInsertTMP-inducible destabilized GAL80 (GAL80-DD)
TagsDDExpressionInsectPromoternSynaptobrevinAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only