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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,236 results for: Sele

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Showing: 21 - 40 of 20236 results

  1. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA(R118G)-HA
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    36047-plasmid-map-sequence-id-381279

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  2. MCS-BioID2-HA

    Plasmid
    #74224
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74224-plasmid-map-sequence-id-238509

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  3. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Publication
    Roux et al J Cell Biol. 2012 Mar 12.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    35700-plasmid-map-sequence-id-228311

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  5. myc-BioID2-MCS

    Plasmid
    #74223
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    Myc and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74223-plasmid-map-sequence-id-334595

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  12. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61357-plasmid-map-sequence-id-358013

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  14. Lamp1-RFP

    Plasmid
    #1817
    Purpose
    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Publication
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Available since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1817-plasmid-map-sequence-id-350820

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  19. mCherry2-N1

    Plasmid
    #54517
    Purpose
    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54517-plasmid-map-sequence-id-352457

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Showing: 21 - 40 of 20236 results