We narrowed to 14,039 results for: TIM

1. pMY-MCS-P2A-eGFP

   Plasmid

   #163356

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SWEET11:SWEET11-2A-GFP-GUS

   Plasmid

   #172484

   Purpose

   Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

   Insert

   SWEET11 (AT3G48740 Mustard Weed)
   

   Tags

   GFP, GUS

   Expression

   Plant

   Promoter

   SWEET11

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV301

   Plasmid

   #204969

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   ble (bleomycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2CG2-QUAS5x:GFPNLS-SV40pA

   Plasmid

   #155122

   Purpose

   Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUAS5x:GFPNLS
   

   Use

   Zebrafish expression

   Promoter

   QUAS5x-E1b

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB2-neo-TET

   Plasmid

   #51287

   Purpose

   expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   sine B2
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   enhancer from 7SL gene

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-N1-A2BR

   Plasmid

   #37202

   Depositor

   Robert Tarran

   Article

   Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

   Insert

   A2BR (ADORA2B Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

   Plasmid

   #115136

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

   Plasmid

   #226011

   Purpose

   CasRx guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP gRNA (BRDN0000562805)

   Plasmid

   #80035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV303

    Plasmid

    #204978

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    amdS
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    two silent mutations (nt177T>C, nt879C>T)

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCyRFP1-RhoA

    Plasmid

    #84358

    Purpose

    fusion protein of RhoA, FRET donor

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1-RhoA (RHOA Synthetic, Human)
    

    Tags

    mCyRFP1 N terminal fusion to RhoA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

    Plasmid

    #134332

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Lmo
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

    Plasmid

    #134340

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC1
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. XE120 CAMKII-K42M 1-271-CS2+

    Plasmid

    #16734

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-K42M 1-271 (Camk2a Rat)
    

    Use

    Xenopus expression

    Mutation

    C-terminal truncation of K42M kinase

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

    Plasmid

    #74289

    Purpose

    pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    H2B-GFP and oG (optimized Glycoprotein)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    Ef1a

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe RFP1-Smad2 neo

    Plasmid

    #58491

    Purpose

    mammalian expression of mRFP1-tagged mouse Smad2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Smad2 (Smad2 Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Available Since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSLGCV

    Plasmid

    #49862

    Purpose

    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

    Depositor

    Anne Glover, Jonathan Pettitt

    Article

    Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

    Insert

    firefly luciferase luc+ fused to GFP (S65C)
    

    Tags

    GFP (S65C)

    Expression

    Worm

    Promoter

    sur-5

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK-Drosha(UniProt)-FLAG-mEGFP

    Plasmid

    #164691

    Purpose

    Express Drosha-FLAG-mEGFP in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    Drosha (DROSHA Human)
    

    Tags

    FLAG and mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP-CD28TMD-NTEVp(H75E)

    Plasmid

    #137829

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75E)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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