We narrowed to 8,320 results for: Set
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Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRTagsExpressionMutationhg38:4:55435551:A>G, hg38:4:55435749:G>APromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC02
Plasmid#114681PurposesfGFP.Strand10-HaloenzymeDepositorInsertsfGFP.Strand10-Haloenzyme
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#230
Plasmid#110925PurposeVector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cordDepositorInsertsunc-129 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
BASIC_10_J23101-RBS32-GFP-B0015
Plasmid#68144Purposestorage vector for BASIC part: GFP expression cassetteDepositorInsertJ23101-RBS32-GFP-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cyEGFP-spo5DSR
Plasmid#49867Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_3b_002_Placeholder
Plasmid#235877PurposeEncodes a sfGFP cassette in the N-tag position used for placeholder cloningDepositorInsertN-tag placeholder
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_5_001_3'UTR_Placeholder
Plasmid#235905PurposeEncodes a sfGFP cassette in the 3'UTR position used for placeholder cloningDepositorInsert3'UTR placeholder
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_4_001_CDS_Placeholder
Plasmid#235892PurposeEncodes a sfGFP cassette in the CDS position used for placeholder cloningDepositorInsertCDS placeholder
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_2_001_Promoter_placeholder
Plasmid#235808PurposeEncodes a sfGFP cassette in the promoter position used for placeholder cloningDepositorInsertPromoter placeholder
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM-FLEX-hM4D(Gi)-mCherry
Plasmid#232782PurposeAAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.DepositorInserthM4D(Gi)-mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF503
Plasmid#222587PurposeBB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertgsdA (gsdA )
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF522
Plasmid#222588PurposeBB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertZWF1
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF8
Plasmid#222592PurposeBB2 gsdA tet-on overexpression cassetteDepositorArticleInsertgsdA
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCF.111
Plasmid#227187PurposepORTMAGE-Ec1 + supplement E. coli SSBDepositorInsertCspRecT, mutL_E32K, E. coli SSB
UseTagsExpressionBacterialMutationmutL E32KPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-Moontag:HA
Plasmid#219648PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest methodDepositorInsertMoonTag:HA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-EYFP:HA
Plasmid#219656PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest methodDepositorInsertEYFP:HA SEED Cassette
UseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKDC.118
Plasmid#227188PurposeT7 IPTG-driven Cas13aDepositorInsertLbuCas13a
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP5
Plasmid#209960PurposeEntry vector to store Level 0* resistance cassette partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC-C-SF-TAP-DEST-TLK1- M566T
Plasmid#218831PurposeExpresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 M566T (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationM566TPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSCL194
Plasmid#222225PurposeExpresses site 1/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 1/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL195
Plasmid#222226PurposeExpresses site 2/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 2/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL368
Plasmid#222227PurposeExpresses site 3/Cas9/Eco1 RT in HIS3 locus.DepositorInsertsite 3/Cas9/Eco1 RT
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.425
Plasmid#203944PurposeUp Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.426
Plasmid#203945PurposeDown Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 2
Plasmid#207610PurposesgRNA 2 to knockout TR by replacement with a PuroR cassetteDepositorInsertTCAGGCCGCAGGAAGAGGAA
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only