We narrowed to 14,348 results for: TIM

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

Plasmid

#169333

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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XE120 CAMKII-K42M 1-271-CS2+

Plasmid

#16734

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-K42M 1-271 (Camk2a Rat)

Use

Xenopus expression

Mutation

C-terminal truncation of K42M kinase

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-p54

Plasmid

#35379

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

p54 (NONO Human)

Tags

FLAG

Expression

Mammalian

Mutation

A464V

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

Plasmid

#115516

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Promoter

UAS

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

ChroME-ST

Use

AAV

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Plasmid

#137830

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Leucine 71 to Lysine in CTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ828

Plasmid

#155322

Purpose

Constitutive expression of PhyB(1-650)-VP16 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15D

Plasmid

#69005

Purpose

mammalian expression of human p53 S15D mutant

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15D

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AlaRS (alaS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-pX330-Cas9-Blast

Plasmid

#159741

Purpose

Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15A

Plasmid

#69004

Purpose

mammalian expression of human p53 S15A mutant.

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Mutation

S15A

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-Drosha(UniProt)-FLAG

Plasmid

#164690

Purpose

Express Drosha-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-Lck-FRB-mTurquoise2

Plasmid

#176130

Purpose

Plasma membrane localized FRB, rapamycin heterodimerization

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

FRB

Use

Lentiviral

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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