We narrowed to 14,039 results for: TIM

1. pFUGW-GtACR1_C102A-EYFP

   Plasmid

   #119196

   Purpose

   Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin GtACR1 step-function mutant C102A
   

   Use

   Lentiviral

   Tags

   EYFP

   Mutation

   changed cysteine 102 to alanine

   Promoter

   Ubiquitin

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fz4-GFP

   Plasmid

   #42197

   Depositor

   Robert Lefkowitz, Jeremy Nathans

   Article

   Chen et al Science. 2003 Sep 5;301(5638):1391-4.

   Insert

   Frizzled 4 (Fzd4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD313

   Plasmid

   #212931

   Purpose

   Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   Sed1 (SED1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTSK561

   Plasmid

   #190816

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

   Depositor

   Tatiana Karpova, Gunjan Mehta

   Article

   Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twitch-3 pcDNA3

   Plasmid

   #49532

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-3
   

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-MCU

   Plasmid

   #31726

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Mutation

   *

   Promoter

   None

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.Flex.Twitch2B.WPRE.SV40

   Plasmid

   #100039

   Purpose

   AAV expression of FLEXed Twitch2B driven by CAG promoter. Twitch 2B is a fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch2B
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2CG2-QUASM1:GFPNLS-SV40pA

   Plasmid

   #155123

   Purpose

   Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:GFPNLS
   

   Use

   Zebrafish expression

   Promoter

   QUASM1-E1b

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

   Plasmid

   #68719

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6f
   

   Use

   AAV

   Promoter

   CAG-FLEX

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. VEGF binding scFv G6

    Plasmid

    #111716

    Purpose

    Yeast Surface Display of G6 as a reference starting point for design

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6
    

    Tags

    cmyc tag

    Expression

    Yeast

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMHE:mTREK-1(tandem wt-wt)

    Plasmid

    #224757

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

    Depositor

    Dan Minor

    Article

    Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

    Insert

    Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCarCBADE_ProB(I69E)A

    Plasmid

    #117756

    Purpose

    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    

    Expression

    Bacterial

    Promoter

    PLac(UV5)

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-NIrD

    Plasmid

    #65010

    Purpose

    NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

    Depositor

    Wensheng Wei

    Article

    Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

    Inserts

    rtTA-MAVS(C)
    2A-linked delta-TK
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Tight-TRE

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pJH4203

    Plasmid

    #200160

    Purpose

    Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    twk-40 (twk-40 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Prig-3

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET-45b-mEGFP

    Plasmid

    #185013

    Purpose

    Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    Sirius-GFP
    

    Expression

    Bacterial

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-7xTcf-NLS-CNL

    Plasmid

    #65712

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL
    

    Use

    Luciferase

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

18. MSCV-myrAkt

    Plasmid

    #49267

    Purpose

    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

    Depositor

    Lawrence Kane, Arthur Weiss

    Article

    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.

    Insert

    Akt (AKT1 Bovine)
    

    Use

    Retroviral

    Tags

    IRES-EGFP and myr

    Expression

    Mammalian

    Mutation

    N2D, D149E and A478G compared to AAW71957.1

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBAD-SPW-RHH

    Plasmid

    #190630

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-RHH

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-RHH
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+/N-Halo

    Plasmid

    #184417

    Purpose

    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    Halo-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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