163,606 results

1. pGW-TurboID-GOI

   Plasmid

   #209380

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry2-N1

   Plasmid

   #54517

   Purpose

   Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Promoter

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. shp53 pLKO.1 puro

   Plasmid

   #19119

   Purpose

   3rd gen lentiviral vector for knocking down p53 gene expression

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Insert

   small hairpin RNA against tumor protein p53 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Use

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET16B.Pfu

   Plasmid

   #12509

   Purpose

   Bacterial expression of Pfu DNA polymerase

   Depositor

   Sung-Hou Kim

   Article

   Goldman et al Acta Crystallogr D Biol Crystallogr. 199

   Insert

   Pfu DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y218H, Q462R, F533L, K559I and V683A (mutations w…

   Promoter

   Available Since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-tadDE-PcrA M6-UGI

   Plasmid

   #221236

   Purpose

   Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   tadDE-PcrA M6-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ M2rtTA_CAGG TetON-Sox10

   Plasmid

   #115240

   Purpose

   RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

   Depositor

   Catherine Verfaillie

   Article

   Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

   Insert

   SOX10 (SOX10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ADAM10-HA

   Plasmid

   #65106

   Purpose

   Expression of HA tagged ADAM10 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Synthetic, Zebrafish)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdTomato-anti-AlfaTag nanobody

    Plasmid

    #171819

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    tdTomato-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1-BFP

    Plasmid

    #120577

    Purpose

    Lentiviral plasmid to co-express a guide RNA and EBFP.

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDESTmycDDX17

    Plasmid

    #19876

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV:cTNT::Luciferase

    Plasmid

    #69915

    Purpose

    Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

    Insert

    Firefly luciferase
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Chicken cardiac troponin T

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-C-TEV 219

    Plasmid

    #100096

    Purpose

    SNIPer plasmid, C terminal TEV fused to FKBP

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    C-TEV
    

    Use

    Retroviral

    Tags

    FKBP and Myc

    Expression

    Mammalian

    Mutation

    C terminal domain

    Promoter

    CMV

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCas9-BFP

    Plasmid

    #78545

    Purpose

    Fusion protein Cas9+BFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. human p53-(82-393)

    Plasmid

    #24867

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 82-393

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-PE6e

    Plasmid

    #207855

    Purpose

    Mammalian expression of PE6e prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6e
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, K918A, K775R)

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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