We narrowed to 13,642 results for: NTS

1. pET28 mid.5

   Plasmid

   #191896

   Purpose

   Encodes a GFP design, mid.5

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.5
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Y145M T167V H181Y L220V V224I

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28 mid.4

   Plasmid

   #191895

   Purpose

   Encodes a GFP design, mid.4

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.4
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V68A S72A Y145M L220V

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 sf:bleach.3

   Plasmid

   #191925

   Purpose

   Encodes a GFP design, sf:bleach.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:bleach.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28 bleach.3

   Plasmid

   #191914

   Purpose

   Encodes a GFP design, bleach.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   bleach.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28 mid.12

   Plasmid

   #191913

   Purpose

   Encodes a GFP design, mid.12

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.12
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S S72V Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28 em-blue-shift.2

   Plasmid

   #191901

   Purpose

   Encodes a GFP design, em-blue-shift.2

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   em-blue-shift.2
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S S72A T108I Y145F V150I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 acid.5

   Plasmid

   #191903

   Purpose

   Encodes a GFP design, acid.5

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.5
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72T Y145M T167V H181V

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 acid.4

   Plasmid

   #191898

   Purpose

   Encodes a GFP design, acid.4

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.4
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V61L Q69L Y145M L220V

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28 acid.3

   Plasmid

   #191897

   Purpose

   Encodes a GFP design, acid.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69L S72A T108V Y145M V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28 slow.2

    Plasmid

    #191894

    Purpose

    Encodes a GFP design, slow.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    slow.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V68M Q69L S72T V112I Y145M T167V L220V V224I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28 sf:mid.10

    Plasmid

    #191934

    Purpose

    Encodes a GFP design, sf:mid.10

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.10
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    L42V Q69L S72A Y145F T167I L220V V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28 bleach.1

    Plasmid

    #191885

    Purpose

    Encodes a GFP design, bleach.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.1
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108L Y145M T167V L220V

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28 mid.3

    Plasmid

    #191884

    Purpose

    Encodes a GFP design, mid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A T108V Y145M T167V H181I V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28 thermo.9

    Plasmid

    #191882

    Purpose

    Encodes a GFP design, thermo.9

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    thermo.9
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T167V T203H E222Q

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28 basic.3

    Plasmid

    #191881

    Purpose

    Encodes a GFP design, basic.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    basic.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S V68A S72A Y145S L220V V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28 mid.2

    Plasmid

    #191880

    Purpose

    Encodes a GFP design, mid.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108I Y145I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28 bleach.6

    Plasmid

    #191879

    Purpose

    Encodes a GFP design, bleach.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.6
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L S72C Y145M H181L V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28 slow.3

    Plasmid

    #191877

    Purpose

    Encodes a GFP design, slow.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    slow.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72V Y145S T167V L220V V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

    Plasmid

    #192373

    Purpose

    To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

    Plasmid

    #192372

    Purpose

    To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge