We narrowed to 16,471 results for: por

1. TB:5xNF- κβ_RE:MiniP

   Plasmid

   #133878

   Purpose

   5 copies of the NF-κβ DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-nuclear-EKAR4

   Plasmid

   #174439

   Purpose

   Nuclear-targeted EKAR4.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   nuclear-EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132399

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDriver

   Plasmid

   #165149

   Purpose

   Driver plasmid for PROBER encoding SV40 Large T-antigen

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Insert

   SV40 Large T-antigen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. dClover2-N1

   Plasmid

   #54538

   Purpose

   Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

   Depositor

   Michael Davidson

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   dClover2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCdrA-gfp(ASV)C

   Plasmid

   #111615

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGGS-MCS-NLS-3XFLAG-V5

   Plasmid

   #196383

   Purpose

   Empty backbone vector to insert cDNA for strong expression in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGs

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

   Plasmid

   #132962

   Purpose

   Firefly-luciferase single-strand annealing assay cloning backbone

   Depositor

   Gang Bao

   Article

   Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

   Insert

   Firefly Luciferase
   

   Use

   CRISPR and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Stop sequence between luciferase gene

   Promoter

   CMV

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132400

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-TPD54

    Plasmid

    #172449

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB4542

    Plasmid

    #215261

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB4401

    Plasmid

    #215252

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-mouse vinculin full length (889)

    Plasmid

    #67935

    Purpose

    Mammalian expression of mouse vinculin with GFP fusion

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L159P in vinculin

    Promoter

    CMV

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPPC019.AAV

    Plasmid

    #171147

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CD63-(TEV)-tTA

    Plasmid

    #162597

    Purpose

    human CD63-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD63 (CD63 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSprayerHE

    Plasmid

    #165150

    Purpose

    Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    BASU-GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1-nv5-ER beta

    Plasmid

    #22770

    Depositor

    Donald McDonnell

    Article

    Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

    Insert

    estrogen receptor beta (ESR2 Human)
    

    Use

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. Arf Q71L CFP

    Plasmid

    #128149

    Purpose

    Expression of Arf Q71L

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 2002 May 9;417(6885):187-93. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q71L

    Promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiPGK Hygro DEST H2B-mCerulean3

    Plasmid

    #90234

    Purpose

    Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLR5-CAG-TetR_W43F-3xmNG

    Plasmid

    #174880

    Purpose

    PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    TetR(W43F)-3xmNeonGreen
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Circular-oc-EMCV-GFP

    Plasmid

    #226261

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LOV-Turbo-V5_pRS415

    Plasmid

    #199667

    Purpose

    expresses LOV-Turbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    Promoter

    Gal1,10

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-His-hAos1

    Plasmid

    #53135

    Purpose

    E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Aos1 (SAE1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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