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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (EGFP-mRFP)

Plasmid

#18680

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Tags

EGFP and mRFP

Expression

Mammalian

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0197

Plasmid

#154654

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS Nanoluc expression cassette [NOS::TMV::LucN::Flag::nosT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR29_00121041

Plasmid

#121233

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR29_00121041

Use

Golden gate

Mutation

Magnaporthe grisea

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-Fzd8

Plasmid

#246709

Purpose

Expression vector for mouse Fzd8, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

Fzd8 (Fzd8 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pISEre1

Plasmid

#226819

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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NanoLuc-AF9 Vector

Plasmid

#238745

Purpose

Express NanoLuc-AF9 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

AF9 (MLLT3 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGiantin-mScarlet3-H (aka mYongHong)

Plasmid

#239409

Purpose

Expresses Giantin-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Giantin (GOLGB1 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pN3-sTRAIL

Plasmid

#154246

Purpose

Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

Depositor

Jordan Green

Article

Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

Insert

Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pVO-NanoLUC

Plasmid

#234900

Purpose

This all-in-one vector is used to overexpress the NanoLUC gene via virus-mediated overexpression (VOX)

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

NanoLUC

Expression

Plant

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSW030

Plasmid

#201317

Purpose

UBI:Eluc (green-shifted luciferase with Ubiquitin promoter

Depositor

Benjamin Schwessinger

Insert

Eluc

Expression

Plant

Mutation

removal of BsaI and BpiI sites

Promoter

pICSL12009

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b

Plasmid

#215820

Purpose

Express CBE6b (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_iRFP670 sgAAVS1

Plasmid

#192477

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A iRFP670

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMD2.G-K47Q/R354Q

Plasmid

#207323

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

K47Q/R354Q

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide Puro-P2A-EGFP

Plasmid

#137729

Purpose

For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

Depositor

Fredrik Wermeling

Article

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAV5S-F30-2xdBroccoli

Plasmid

#66845

Purpose

Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Tags

5S rRNA

Expression

Mammalian

Promoter

5S

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3

Plasmid

#73021

Purpose

Expresses BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE3

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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