We narrowed to 10,763 results for: ESP
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Plasmid#22924DepositorInserthuman ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gs
Plasmid#109350PurposeWildtype GαsDepositorAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(-) mouse Egr3
Plasmid#107998PurposeExpresses mouse Egr3 in mammalian cellsDepositorAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2
Plasmid#195526PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoterDepositorInsertAce-mNeon2
UseAAVExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
COC053
Plasmid#209131PurposeEnzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.DepositorInsertEnzyme BirA (birA E. coli)
ExpressionBacterialAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSPromoterUASAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD_sfGFP3TCG4TAG
Plasmid#174518PurposeRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.DepositorInsertsfGFP
Tags6 x HisExpressionBacterialMutationRecoded. TCG and TAG codons were introduced in po…PromoteraraBADAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorExpressionMammalianPromoterTet responsive elementAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pIRES_IBB-mCherry-IRES-HA-mAID-nanobody
Plasmid#117720PurposeExpression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNADepositorInsertsmAID-nanobody
IBB-mCherry
Tagsimportin-beta bining domain (IBB) and triple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMV and None, IRES-drivenAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Ace-mNeon2
Plasmid#195518PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of synapsin promoterDepositorInsertAce-mNeon2
UseAAVExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterSynapsinAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialPromoterccaR and cpcG2Δ59Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-REDD1-HA
Plasmid#133550PurposeExpresses HA tagged REDD1 in Mammalian cellsDepositorInsertRegulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
UseRetroviralTagsHA tagExpressionMammalianAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE mutant-Luc pGK Hygro
Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2-Kv2.1PR
Plasmid#195524PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
TagsnoneExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-NMHC-IIAΔtailpiece
Plasmid#35689DepositorInsertMHC IIA (MYH9 Human)
TagsEGFPExpressionMammalianMutationAdded stop codon after codon 1928, eliminating th…PromoterCMVAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic Mut E2
Plasmid#48748PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseMutationMutated E-box2 sequence from CACGTT to GCTAGTAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-EGFP_puromycin
Plasmid#65284PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5xNF-kb_RE
Plasmid#118050PurposeGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-VARNAM2-Kv2.1PR
Plasmid#195533PurposeCre-dependent expression of a red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAV and Cre/LoxExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBRPyCAG-hKlf2-DsRed-Ip
Plasmid#26277DepositorInsertKlf2 (KLF2 Human)
ExpressionMammalianAvailable SinceNov. 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro) mouse Egr2
Plasmid#108097PurposeRetroviral vector for expression of mouse Egr2DepositorAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBK.134
Plasmid#187219PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectivelyDepositorInsertA: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseSynthetic BiologyExpressionBacterialPromoterA: pTet*; B: pBetIAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-VARNAM2-Kv2.1PR
Plasmid#195530PurposeRed fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterCaMKIIAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-VARNAM2-Kv2.1PR
Plasmid#195536PurposeFlp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-pAceR-Kv2.1PR
Plasmid#195525PurposeRed fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertpAceR-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM 78E, 81D, 92N; SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rec-PhoA/CsgA
Plasmid#170787PurposeRecombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinaseDepositorUseSynthetic BiologyTagsHHHHHHExpressionBacterialPromoterInverted proD with Bxb1recognition sites and Inve…Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro) mouse Egr3
Plasmid#108102PurposeRetroviral vector for expression of mouse Egr3DepositorAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA-TRE
Plasmid#225336PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiExpressionMammalianPromoterTRE (tetracycline-responsive element)Available SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTon1(acgfp1)
Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-dsRed-L3 #BGV203
Plasmid#48973PurposeSupplies the selection marker (dsRed) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertdsRed
UseGateway cloning vectorTagsIRES (strong)PromoternoneAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-IFITM3-F8-TAG
Plasmid#122475Purposeexpresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.DepositorInsertIFITM3 (IFITM3 Human)
TagsHAExpressionMammalianMutationChanged Phenylalanine 8 to amber codonAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iBlast-L3 #BGV204
Plasmid#48982PurposeSupplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertblasticidin-S deaminase
UseGateway cloning vectorTagsIRES (weak)PromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseMutationMutated E-box2 sequence from CACGTT to GCTAGTAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-mCherry_puromycin
Plasmid#65285PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iHygro-L3 #BGV206
Plasmid#48985PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInserthygromycin phosphotransferase
UseGateway cloning vectorTagsIRES (weak)PromoternaAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6CNW)RS
Plasmid#222873PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4xSMAD_RE
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…ExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 gamma (agf)
Plasmid#20354DepositorInsertpDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
TagsFlagExpressionMammalianMutationReplace Thr 183 and Tyr 185 with Ala and Phe resp…Available SinceApril 29, 2009AvailabilityAcademic Institutions and Nonprofits only