161,123 results

1. pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

   Plasmid

   #121538

   Purpose

   An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

   Depositor

   Christopher Richie

   Article

   Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

   Has Service

   AAV2 and AAV5

   Insert

   HA-tagged hM4D(Gi) (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SYN1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-dCas12a-ABE7

   Plasmid

   #193644

   Purpose

   Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-TadA-TadA*7.10, EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. DD-DamV133A-tdTomato-LMNB1

   Plasmid

   #159604

   Purpose

   Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamV133A-tdTomato-LMNB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dam V133A mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXD71Cas10.0-Pct5.1-crRNA(AarI)

   Plasmid

   #191633

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   CymR
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SpyTagMBP

   Plasmid

   #35050

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyTagMBP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Simple peptide tag added to the N-terminus of MBP…

   Promoter

   T7 promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe NEO mRFP1

   Plasmid

   #98397

   Purpose

   Retroviral vector for constitutive mRFP1 expression

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   mRFP1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiGuide

   Plasmid

   #117986

   Purpose

   pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

   Depositor

   Paul Khavari

   Article

   Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

   Insert

   sgRNA backbone (F+E)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL-mU6-GFPGO2-IRES-mScarletI

   Plasmid

   #136896

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   2xNLS on GFP, 3xNLS on mScarlet

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-GFPLaminA-N195K

   Plasmid

   #32708

   Depositor

   Wendy Bickmore

   Article

   Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N195K

   Promoter

   CMV

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CaMKII-WHaloCaMP1a-EGFP

    Plasmid

    #205309

    Purpose

    Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. myc-Rictor corrected

    Plasmid

    #11367

    Purpose

    Mammalian expression of myc tagged human RICTOR

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-LK03

    Plasmid

    #206512

    Purpose

    AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

    Depositor

    Mark Kay

    Article

    Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

    Insert

    LK03 capsid
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    p5

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH1R-mCherry-N1

    Plasmid

    #84325

    Purpose

    Histamine-1 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H1R (HRH1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-PDE4DCatL
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-CreERT2 stuffer v3

    Plasmid

    #158046

    Purpose

    lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. RSV-Flag-Brd3

    Plasmid

    #86615

    Purpose

    Flag tagged mouse Brd3 expression construct for cell transfection

    Depositor

    Mario García-Domínguez

    Article

    Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

    Insert

    Brd3 (Brd3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CBh-IgKL-mNG

    Plasmid

    #183467

    Purpose

    To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid

    #197500

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid

    #164193

    Purpose

    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    F391W-L398V

    Promoter

    Syn

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GPER-DuET

    Plasmid

    #213256

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPER (GPER1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. CMV::SypHy A4

    Plasmid

    #24478

    Depositor

    Leon Lagnado

    Article

    Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

    Insert

    SypHy (Syp Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDEST17-UbE2B

    Plasmid

    #15781

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2B (UBE2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3-GFPLaminA-L85R

    Plasmid

    #32707

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L85R

    Promoter

    CMV

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pS2513·PHP

    Plasmid

    #122590

    Purpose

    Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

    Depositor

    Pablo Ivan Nikel

    Article

    Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

    Insert

    phIorine2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-CreERT2

    Plasmid

    #14797

    Purpose

    Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSET-B mCherry

    Plasmid

    #108857

    Purpose

    Encodes for the fluorescent protein mCherry

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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