We narrowed to 16,471 results for: por

1. AAVS1-PuroR Xlone eGFP

   Plasmid

   #179837

   Purpose

   Doxycycline-inducible expression of eGFP

   Depositor

   Xiaoping Bao

   Article

   Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN ; Donor plas…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. DsRed-rab9 DN

   Plasmid

   #12676

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB9 (Homo sapiens) (RAB9A Human)
   

   Use

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   changed serine 21 to asparagine

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-MyosinVb-Ctail

   Plasmid

   #110170

   Purpose

   Dominant negative effect by competing with MyosinVb C-terminal tail interactions

   Depositor

   Jose Esteban

   Article

   Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

   Insert

   Myosin Vb (Myo5b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   amino acids 1262-1670

   Promoter

   CMV

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAD-CMV-STAT5A-CA-CMV-GFP

   Plasmid

   #83255

   Purpose

   Adenoviral expression of constitutively active STAT5A with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

   Inserts

   Stat5a (Stat5a Mouse)
   eGFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   constitutively active

   Promoter

   CMV

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP-GFP-FAPP(PHD)

   Plasmid

   #221748

   Purpose

   Stable expression of GFP-FAPP(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   FAPP (PLEKHA3 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–101

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA-DAB2

   Plasmid

   #162690

   Purpose

   Expresses DabAB2 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB2 inorganic carbon transport complex
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO-OMOR-eYFP

   Plasmid

   #66847

   Purpose

   Expresses Opto-MOR in a Cre dependent manner

   Depositor

   Michael Bruchas

   Article

   Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

   Insert

   Opto Mu opioid receptor
   

   Use

   AAV and Cre/Lox

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

   Plasmid

   #45579

   Purpose

   Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani K227M
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-FRB-mCherry-MinD-IRES-Puro

    Plasmid

    #213116

    Purpose

    Expression of FRB-mCherry-MinD in mammalian cells for payload delivery

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    FRB-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBait-YY1

    Plasmid

    #165147

    Purpose

    Bait plasmid for PROBER with YY1-binding site (positive control)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    3X YY1 motif
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1-Lck-GCaMP3

    Plasmid

    #26974

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

    Insert

    Lck-GCaMP3
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-eCas9-sgNT

    Plasmid

    #140238

    Purpose

    Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKanCMV-CX4.3-7aa-mRuby3

    Plasmid

    #74254

    Purpose

    Mammalian expression of C-terminal fusion of mRuby3 to CX43

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    Connexin 43
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and CMV

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. CMV-PKCa-mEGFP-N1

    Plasmid

    #112269

    Purpose

    IDOCKSalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    Protein Kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLYS1-EMRE/RNAi resistant

    Plasmid

    #50056

    Purpose

    Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    EMRE-RNAi resistant (SMDT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RNAi resistant, silent mutant

    Promoter

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDAS12342_PEAR-GFP_2in1

    Plasmid

    #177184

    Purpose

    PEAR-GFP plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. Teto-FUW-Pax6

    Plasmid

    #61541

    Purpose

    Expresses Pax6 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Pax6 (Pax6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

23. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXs_FLAG-NMNAT2

    Plasmid

    #133260

    Purpose

    Express FLAG-tagged NMNAT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT2 (NMNAT2 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-GfaABC1D-GRAB_eCB2.0

    Plasmid

    #164609

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    GfaABC1D

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pATP403red-hENT1

    Plasmid

    #166027

    Purpose

    Expresses human equilibrative nucleoside transporter 1 (hENT1)

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    human equilibrative nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTDH3

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-CD81-(TEV)-tTA

    Plasmid

    #162598

    Purpose

    human CD81-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD81 (CD81 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDONR221_SLC22A1

    Plasmid

    #132034

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A1 (SLC22A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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