We narrowed to 12,284 results for: BASE
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Plasmid#169550PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertcHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2343_Tier3(SB)-YPet_Blast
Plasmid#169641PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2344_Tier3(SB)-mRuby2_Blast
Plasmid#169642PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64
Plasmid#169597PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).DepositorInsertdead S.pyogenes Cas9 - VP64 fusion
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2339_Tier3(SB)-Ruby_Puro
Plasmid#169637PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2354_Tier3(PB)-Ruby_PuroR
Plasmid#169653PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mRuby2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2357_Tier3(PB)-TagBFP_BlastR
Plasmid#169656PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mTagBFP2 marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-BlastR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and BlastR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH663
Plasmid#169607PurposeTier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
UseTagsExpressionMammalianMutationPromotertetO7-PCMVmin / PPGK / PPGKAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH19-Tier1-PhCMV-p300core
Plasmid#169572PurposeTier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)DepositorInsertPCMV-driven P300core (EP300 Human)
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2304-Tier1-Tactb-PPGK-NLuc_TB
Plasmid#169549PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertTactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2380-Tier1-PhCMV-TM
Plasmid#169485PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).DepositorInsertPCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-50-DExogron-mCherry-R11b
Plasmid#179904PurposeDonor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKRQ_11_DExCon-mNeonGreen-R25
Plasmid#179896PurposeDonor with homologous arms for Rab25 to knock in DExCon-mNeonGreen moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-23-DExCon-mCherry-R11b
Plasmid#179898PurposeDonor with homologous arms for Rab11b to knock in DExCon-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-67-DExCon-mNeonGreen-R11b
Plasmid#179899PurposeDonor with homologous arms for Rab11b to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-59-DExCon-mTagBFP2-R25
Plasmid#179900PurposeDonor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:TBA2p
Plasmid#128429PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.DepositorInsertTESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
UseGateway promoter entry cloneTagsExpressionMutationPromoterTESTA ABUNDANT 2Available sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-mEKLF Zinc Fingers
Plasmid#67837PurposeExpression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
UseTagsFlagExpressionMammalianMutationZinc Fingers are amino acids 287-end; this number…PromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-Luc
Plasmid#63697Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsLuciferaseExpressionMammalianMutationPromoterTATA boxAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFM038
Plasmid#40141DepositorInsertpar-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + 7SA; base pairs 6-523 of par-2 were re…Promoterpie-1Available sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFM041
Plasmid#40144DepositorInsertpar-2 RNAi resistant (S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationS241A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM039
Plasmid#40142DepositorInsertpar-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + S241A; base pairs 6-523 of par-2 were …Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM036
Plasmid#40139DepositorInsertpar-2 RNAi resistant (K162A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationK162A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM052
Plasmid#40146DepositorInsertpar-2 RNAi resistant (C56S) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationC56S; base pairs 6-523 of par-2 were recoded to …Promoterpie-1Available sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM037
Plasmid#40140DepositorInsertpar-2 RNAi resistant (R163A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR163A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pM-ABE
Plasmid#196292Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding ABE in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationSee depositor comments belowPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiFUCCI(CA)5
Plasmid#223176PurposeLentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)DepositorInsertFUCCI(CA)5
UseLentiviralTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKSE401
Plasmid#62202PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
p026 pCS2-EnR
Plasmid#11028DepositorInsertengrailed (en Fly)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationEngrailed repressor domain (bases encoding amino …PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
Plasmid#192939PurposeFlpO-based digitizer circuit driven by AP1 promoterDepositorInsertsAP1(2)/minTK promoter
FlpO-ABI
PYL-FlpO
CAG promoter
frt-STOP-frt
GFP
luciferase
pPGK
Zeocin resistance
BFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-ECC-Biosensor
Plasmid#140832PurposeEncoding the CAG promoter based eccDNA biosensorDepositorInsertECC-Biosensor
UsePiggybac (pb) transposonTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-TRE-ECC-Biosensor
Plasmid#140833PurposeEncoding the TRE promoter based eccDNA biosensorDepositorInsertTRE-ECC Biosensor
UsePiggybac (pb) transposonTagsExpressionMammalianMutationPromoterinverseAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UUA)_EF1_MmaPylRS
Plasmid#174896PurposeMma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT(UCA)_EF1_MmaPylRS
Plasmid#174899PurposeMma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKPI-MtLAP1
Plasmid#193521PurposeAnthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula rootsDepositorInsertsMtLAP1
DsRed
UseTagsExpressionPlantMutationPromoterATUBI10 and Medtr8g059790(KPI)Available sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only