We narrowed to 4,072 results for: p2a

1. pKISox1-P2A-eGFP-Hygro

   Plasmid

   #115685

   Purpose

   Knock-in of P2A-eGFP at the mouse Sox1 gene

   Depositor

   David Suter

   Article

   Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

   Insert

   P2A-eGFP-loxP-PGK-Hygro-loxP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGS54 (AID-P2A-Hygromycin)

   Plasmid

   #126583

   Purpose

   An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AID-P2A-HygR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-P2A-APEX2-ChBP

   Plasmid

   #108879

   Purpose

   Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   EGFP-P2A-APEX2-ChBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FNLSNG-P2A-Puro

   Plasmid

   #136900

   Purpose

   Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mKate2-P2A-APEX2-csGBP

   Plasmid

   #108875

   Purpose

   Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   mKate2-P2A-APEX2-csGBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMY-MCS-P2A-CD90.2

   Plasmid

   #163355

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-APEX2-P2A-EGFP

   Plasmid

   #108896

   Purpose

   Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-P2A-EGFP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-BirA-P2A-mCherry

   Plasmid

   #74056

   Purpose

   retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   BirA biotin ligase
   

   Use

   Retroviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTRs

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

   Plasmid

   #169558

   Purpose

   Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PSV40-driven citrine-P2A-SEAP fusion construct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PSV40

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMY-MCS-P2A-eGFP

    Plasmid

    #163356

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK463 (BSD-P2A-mAB)

    Plasmid

    #214389

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-P2A-BSD

    Plasmid

    #174819

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PE2-P2A-BSD
    

    Use

    Tags

    P2A-BSD and SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMLV-EBFP-P2A-Puro

    Plasmid

    #160227

    Purpose

    Expresses EBFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EBFP-P2A-Puro
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-H1-shSRGAP2A-hsynapsin-EGFP

    Plasmid

    #220798

    Purpose

    shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

    Plasmid

    #206956

    Purpose

    AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    mScarlet-I, MmMT3, QtEnc
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mKate2-P2A-APEX2-2xFYVE_hrs

    Plasmid

    #67663

    Purpose

    Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-2xFYVE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

    Plasmid

    #232122

    Purpose

    A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    Tcf4ΔN (Tcf4 Mouse)
    

    Use

    Gateway cloning

    Tags

    myc

    Expression

    Mutation

    lacks aa 1-56

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLC-ZsGreen-P2A-Puro

    Plasmid

    #124302

    Purpose

    Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-SaPE2-P2A-BSD

    Plasmid

    #174818

    Purpose

    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2-P2A-BSD
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mKate2-P2A-APEX2-OSBP

    Plasmid

    #67664

    Purpose

    Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, [marks PI4P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-OSBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMVlac-dirTopo-P2A-aGFP

    Plasmid

    #61972

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLC-ZsGreen-P2A-Blast

    Plasmid

    #123322

    Purpose

    Lentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG-RFP-p2a-postASAP

    Plasmid

    #178793

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with RFP

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and TagRFP
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC-dirTopo-P2A-aGFP

    Plasmid

    #61974

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK486 (Hygro-P2A-2mAID)

    Plasmid

    #214398

    Purpose

    2mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-2mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV2-GCaMP6f-P2A-PAmCherry

    Plasmid

    #133412

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pINDUCER-21-RUNX1-P2A-ERG

    Plasmid

    #97045

    Purpose

    Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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