We narrowed to 8,683 results for: sgRNA

1. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-sgRNA_Dgcr8_3

   Plasmid

   #73527

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pX330-sgRNA_Dgcr8_4

   Plasmid

   #73528

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

5. PB_rtTA_BsmB-HNRNPK_sgRNA_4

   Plasmid

   #242899

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_rtTA_BsmB-HNRNPK_sgRNA_1

   Plasmid

   #242897

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB_rtTA_BsmB-HNRNPK_sgRNA_3

   Plasmid

   #242898

   Purpose

   PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

   Insert

   HNRNPK (Hnrnpk Mouse)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA63_sgRNA-B0_M13ps

   Plasmid

   #231421

   Purpose

   M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-B0
   

   Use

   Synthetic Biology

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA63_sgRNA-B1_M13ps

   Plasmid

   #231422

   Purpose

   M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-B1
   

   Use

   Synthetic Biology

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX330-sgRNA006_CTCF

    Plasmid

    #232928

    Purpose

    Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9 (Ctcf S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-sgRNA047_Podxl

    Plasmid

    #232932

    Purpose

    Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Podxl S. pyogenes)
    

    Expression

    Mammalian

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. esgRNA_NbATML1-1pro

    Plasmid

    #231151

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbATML1-1pro
    

    Expression

    Plant

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. esgRNA_NbSTM-5'UTR

    Plasmid

    #231150

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbSTM-5?UTR
    

    Expression

    Plant

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEVA19_sgRNA-C1_M13ps

    Plasmid

    #231411

    Purpose

    M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid

    #232727

    Purpose

    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

    Depositor

    David Brafman

    Article

    Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

    Insert

    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVA26_sgRNA-2_M13ps

    Plasmid

    #231412

    Purpose

    M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEVA26_sgRNA-A0_M13ps

    Plasmid

    #231413

    Purpose

    M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    sgRNA-A0
    

    Use

    Synthetic Biology

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEVA19_LacI_pLac-sgRNA-1

    Plasmid

    #231408

    Purpose

    sgRNA-1 expression from an IPTG-inducible promoter.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    sgRNA-1
    LacI
    

    Use

    Synthetic Biology

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA19_AraC_pBAD-sgRNA-1

    Plasmid

    #231407

    Purpose

    sgRNA-1 expression from an arabinose-inducible promoter.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    sgRNA-1
    AraC
    

    Use

    Synthetic Biology

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA26_sgRNA-A1_M13ps

    Plasmid

    #231414

    Purpose

    M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-A1
    

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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