We narrowed to 73,945 results for: LAS

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pCDH-puro-Bcl-XL

Plasmid

#46972

Purpose

lentiviral expression of Bcl-XL

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-GFP

Plasmid

#215489

Purpose

Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hIRAK1

Plasmid

#180405

Purpose

lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

IRAK1 (IRAK1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-opEBNA2-IRES-GFP

Plasmid

#220354

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

3 x HA tag

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8.20-d

Plasmid

#136301

Purpose

expresses ABE8.20-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-µLight

Plasmid

#201225

Purpose

Express muLight biosensor in mammalian cells

Depositor

Lin Tian

Article

Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

Insert

µLight

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP-N3-EPAC1

Plasmid

#113110

Purpose

Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

Depositor

Xiaodong Cheng

Article

Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

Insert

RapGEF3 (RAPGEF3 Human)

Tags

EYFP

Expression

Mammalian

Promoter

PCMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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BM00654

Plasmid

#155339

Purpose

Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PEmax_AAVS1_knockin_HDR_donor

Plasmid

#223000

Purpose

HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PEmax

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-NE

Plasmid

#215486

Purpose

Lentiviral expression of human alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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BM00369

Plasmid

#155338

Purpose

Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-myr-HA-Akt1

Plasmid

#46969

Purpose

lentiviral expression of myr-HA-Akt1

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

HA and myr

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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