We narrowed to 13,228 results for: 109

1. pEntry_HCoV-OC43-Spike

   Plasmid

   #168951

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-OC43-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCVhyg-H3.3K4M-FLAG/HA

   Plasmid

   #128741

   Purpose

   Expression of histone H3.3K4M-FLAG/HA

   Depositor

   Kai Ge

   Article

   Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

   Insert

   H3.3K4M (H3-3A Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mutation

   K4M

   Promoter

   MSCV

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. QUIN

   Plasmid

   #184267

   Purpose

   A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.

   Depositor

   Feng Qu

   Article

   Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

   Insert

   CPSMV RNA1 cDNA with 4 introns
   

   Use

   Tags

   Expression

   Plant

   Mutation

   S1098P, K1651R

   Promoter

   CaMV 35S with duplicated enhancer

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEntry_HCoV-HKU1-Spike

   Plasmid

   #168911

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-HKU1-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCF1148

   Plasmid

   #222330

   Purpose

   Fluorescent reporter plasmid for Veillonella

   Depositor

   J Christopher Fenno

   Article

   Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

   Insert

   bs2 gene with Veillonella parvula SKV38 mdh promoter
   

   Use

   E. coli-veillonella shuttle plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Veillonella parvula SKV38 mdh promoter

   Available since

   Oct. 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLVX-opto-mCasp8

   Plasmid

   #208782

   Purpose

   Inducibly expresses mouse caspase8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mCaspase8 Cry2 (Casp8 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-V5-KDM3A

   Plasmid

   #82331

   Purpose

   lentiviral expression of KDM3A

   Depositor

   Luke Gaughan

   Article

   Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

   Insert

   KDM3A (KDM3A Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   two silent mutations to make it siRNA resistant

   Promoter

   Available since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AY27_pU6.gRNA.CLYBL

   Plasmid

   #199238

   Purpose

   Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. pyogenes gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRExT2A-NT-PmNG

    Plasmid

    #232200

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPRv2 NT sgRNA3

    Plasmid

    #164929

    Purpose

    Expresses Non-targeting sgRNA3 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA3 (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW1659

    Plasmid

    #154314

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG/hArf4(WT)-mCherry

    Plasmid

    #79406

    Purpose

    Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

17. beta Synuclein-VC

    Plasmid

    #89484

    Purpose

    expresses human beta Synuclein fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJW1816

    Plasmid

    #154320

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEntry_HCoV-229E-Spike

    Plasmid

    #168971

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. talin1-head

    Plasmid

    #182996

    Purpose

    expression of talin1 head wt in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    talin1 head (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRDA_355

    Plasmid

    #187159

    Purpose

    Lentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNA

    Depositor

    David Root, John Doench

    Article

    Aquilanti et al Neuro Oncol. 2023 Jul 6;25(7):1275-1285.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-ABE7.10-P2A-GFP

    Plasmid

    #162509

    Purpose

    Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

    Insert

    ABE7.10-P2A-GFP
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

    Plasmid

    #211767

    Purpose

    dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pGK and pEF1a

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEntry_SARS-CoV-1-Spike

    Plasmid

    #168864

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-1-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP

    Plasmid

    #232754

    Purpose

    Expresses EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cas9-sgRNA-A+B

    Plasmid

    #149370

    Purpose

    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRedCmOcr

    Plasmid

    #176226

    Purpose

    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB and PL

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSMP-EZH2_1

    Plasmid

    #36387

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH2 (EZH2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEntry_HCoV-OC43-nsp12

    Plasmid

    #168949

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp12 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. VN-beta Synuclein

    Plasmid

    #89483

    Purpose

    expresses human beta Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, PGK, and U6

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. KHBD00532

    Plasmid

    #39630

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3629 (Dll Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

36. H9 pMalLP

    Plasmid

    #41811

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    H9 Super-2 (IL2 Human)
    

    Use

    Tags

    3C protease site, 6x His, and Maltose Binding Pro…

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Jan. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pEntry_SARS-CoV-2-Nucleocapsid

    Plasmid

    #168886

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-2-Nucleocapsid (N )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28sumo-MDA5-Full-length

    Plasmid

    #210826

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

    Insert

    MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLC-242

    Plasmid

    #73189

    Purpose

    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    chloramphenicol promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Myc-Bnip3FL

    Plasmid

    #100796

    Purpose

    Mammalian expression of Bnip3 full length splice variant

    Depositor

    Joseph Gordon

    Article

    Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

    Insert

    mus Bnip3 (Bnip3 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LLP777_SnoopCatcher-p65HSF1-CO

    Plasmid

    #211770

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-p65HSF1
    

    Use

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LLP775_αGCN4-p65HSF1-CO

    Plasmid

    #211768

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-p65HSF1
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138130

    Purpose

    Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pscAluYNF1

    Plasmid

    #50934

    Purpose

    Expresses scAluY RNA in mammalian cells.

    Depositor

    Katharina Strub

    Article

    Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

    Insert

    scAluYNF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    the poly(A) linker and the right arm of the dimer…

    Promoter

    7SL RNA promoter (pol III)

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

45. pEntry_MERS-CoV-Spike

    Plasmid

    #168837

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    MERS-CoV-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

    Plasmid

    #120301

    Purpose

    AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

    Plasmid

    #179078

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge