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LRG2.1-mOrange

Plasmid

#124772

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

Plasmid

#204810

Purpose

Bacterial expresison of Enterovirus A71 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

A71 2A protease

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204817

Purpose

Bacterial expresison of Enterovirus D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV D68 3C protease

Expression

Bacterial

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pR_Lux

Plasmid

#226457

Purpose

lambda immunity region to the luciferase operon (lux)

Depositor

Emily Balskus

Article

Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

Insert

lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens

Expression

Bacterial

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pL3-TRE-LucGFP-2L

Plasmid

#11685

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Insert

Luciferase-EGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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LV-Floxed Luc

Plasmid

#60622

Purpose

Expresses a conditional luciferase cassette dependent on Cre mediated recombination

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Floxed Luciferase

Use

Cre/Lox, Lentiviral, and Luciferase

Promoter

human UbC

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDest eGFP CUGBP1

Plasmid

#61276

Purpose

Mammalian expression of EGFP-tagged human CUGBP1

Depositor

Nicolas Charlet-Berguerand

Insert

CUG-BP (CELF1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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mNP-Luc (PPARdelta promoter)

Plasmid

#16532

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

PPAR delta promoter NP (PPARD Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant binding sites

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
PP78

Plasmid

#78948

Purpose

Supplemental Figure 3. Integration Site 5.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 5

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3-3xBoxB-MS2

Plasmid

#136011

Purpose

3 repeats of BoxB and MS2 hairpins inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid for the Tethering assay

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

No gene (Artificial)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1C-YFP-shSCR

Plasmid

#139647

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

See Depositor comments

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels

Plasmid

#204801

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSK-c-mpl

Plasmid

#1828

Depositor

Philip Leder

Article

Skoda et al EMBO J 1993 Jul;12(7):2645-53.

Insert

c-mpl (Mpl Mouse)

Expression

Bacterial

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pBD-0016

Plasmid

#12345

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R313K (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R313K, I206V*(see below)

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
PP74

Plasmid

#78944

Purpose

Supplemental Figure 3. Integration Site 1.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 1

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI

Plasmid

#50719

Purpose

expression of human TPI1 in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Promoter

GPD

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTHSSd_18

Plasmid

#59953

Purpose

Quad split (at 61, 179, 601-SZ)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Four-fragment T7* RNA polymerase

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDB2

Plasmid

#18954

Depositor

Michele Calos

Article

Keravala et al J Gene Med. 2006 Aug . 8(8):1008-17.

Insert

phiC31 attB

Expression

Mammalian

Available Since

Oct. 14, 2008

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBS mScx (CT#404)

Plasmid

#13958

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (Scx Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

Plasmid

#214462

Purpose

Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PIP5K1A (PIP5K1A Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-pomMx1

Plasmid

#30536

Purpose

Reporter (luciferase) for interferon signalling in fish

Depositor

Bertrand Collet, Christopher Secombes

Article

Collet et al Dev Comp Immunol. 2004 Jun . 28(7-8):793

Insert

rainbow trout Mx 1 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-PM-34a-K1/2

Plasmid

#25800

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

alternative hsa-miR-34a promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pAS007

Plasmid

#164868

Purpose

This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phiKO2 operators

Use

Synthetic Biology

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRF TpC RE8

Plasmid

#171047

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE8

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE8

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBS cScx 3'UTR (CT#485)

Plasmid

#13957

Depositor

Cliff Tabin

Article

Schweitzer et al Development. 2001 Oct . 128(19):3855-66.

Insert

Scleraxis (SCX Chicken)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pGC1853

Plasmid

#44619

Depositor

Robert Oshima

Article

Kulesh et al Mol Cell Biol. 1988 Apr;8(4):1540-50.

Insert

keratin 18 gene (KRT18 Human)

Use

Cloning

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only

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