19,773 results

Showing: 6361 - 6390 of 19773 results

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Luciferase:Tnos (GB0110)

Plasmid

#68216

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJYS1Peftu

Plasmid

#85546

Purpose

Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

etfu and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTEC15

Plasmid

#30174

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Wasabi

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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Cav1-GFP

Plasmid

#14433

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

EGFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pHBV-S1-Luc

Plasmid

#71416

Purpose

To see S1 promoter activity of HBV for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S1 promoter driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S1

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC3

Plasmid

#25032

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

EDC3 (EDC3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pC0041-RanCas13b crRNA backbone

Plasmid

#103852

Purpose

For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-RanCas13b DR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCX-OKS-2A

Plasmid

#19771

Purpose

Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

Depositor

Shinya Yamanaka

Article

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)

Expression

Mammalian

Mutation

Three genes are connected with the foot-and-mouth…

Available Since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDP-43

Plasmid

#190093

Purpose

Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

TAR DNA-Binding Protein 43 (TARDBP Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p23-NLS-RfxCas13d-mcherry-NLS-Flag

Plasmid

#165077

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer1

Plasmid

#14763

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-puro /BRAF-V600E

Plasmid

#131724

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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Tagged-Cap

Plasmid

#35572

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Type

Empty backbone

Use

Luciferase

Tags

Ms2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-LUC2CP/intron/ARE

Plasmid

#62858

Purpose

splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

Depositor

Gideon Dreyfuss

Article

Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

insertion of chimeric intron at nueotide poclsiti…

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pICE-RNaseHI-WT-NLS-mCherry

Plasmid

#60365

Purpose

Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

RNase HI

Tags

NLS from SV40 T antigen and mCherry

Expression

Mammalian

Promoter

CMV-tet

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHBV-S2-Luc

Plasmid

#71417

Purpose

To see HBV S2 promoter activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S2 promoter driving luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S2

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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2X PRE TK luc

Plasmid

#11350

Depositor

Donald McDonnell

Article

Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

Insert

PRE

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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19,773 results