59,540 results

1. pcDNA3.1-hRXRα

   Plasmid

   #135910

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha (RXRA Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_hTGM2-Flag

   Plasmid

   #180407

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human USP7-3X FLAG

   Plasmid

   #225334

   Purpose

   Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 (USP7 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Epac-S-H187

   Plasmid

   #170348

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAuroraA-GFP-AURKA

   Plasmid

   #99877

   Purpose

   Expression of GFP-AURKA in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   AURKA minimal

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-KD LKB1

   Plasmid

   #8591

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-sfCherry11x4-ß-actin

   Plasmid

   #70220

   Purpose

   Expresses sfCherry11x4-ß-actin in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry11x4-actin (ACTB Human)
   

   Tags

   sfCherry11x4

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-APOBEC1-YTHmut

    Plasmid

    #131637

    Purpose

    Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    CMV

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0801

    Plasmid

    #141707

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7xE-Box::Renilla

    Plasmid

    #124532

    Purpose

    c-Myc Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    c-Myc Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-myc-HLA-A2

    Plasmid

    #135504

    Purpose

    Mammalian expression of myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MELOPS

    Plasmid

    #80151

    Purpose

    Genetically encoded melanosome-localized pH sensor.

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    OCA2 (OCA2 Human)
    

    Tags

    mNectarine in first luminal loop

    Expression

    Mammalian

    Mutation

    nonpathologic polymorphism D257A, pore mutation V…

    Promoter

    CMV

    Available Since

    Sept. 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid

    #183831

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    L1196M/G1202R

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PD-1-miSFIT-Ce67

    Plasmid

    #124680

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-NPC1L1_STOP

    Plasmid

    #161469

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1L1 (NPC1L1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Tags

    Flag and Ha

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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