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pAAV-CAG-TagRFP658-P2A-GFP

Plasmid

#178971

Purpose

Expresses TagRFP658 and GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658-P2A-GFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSV40_HA-mPACT

Plasmid

#120537

Purpose

protein expression

Depositor

Petr Svoboda

Insert

PACT (Prkra Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pN3-Sp2FL

Plasmid

#107718

Purpose

Expression plasmid for full length murine Sp2

Depositor

Guntram Suske

Insert

Sp2 (Sp2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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RCAS(A) mdnLef1en (CT#1144)

Plasmid

#14022

Depositor

Cliff Tabin

Insert

Lef1 (Lef1 Mouse)

Use

Retroviral; Avian expression

Mutation

Dominant negative Lef1, engrailed fusion

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
FUW-Flag-Mbd3 delta1-70

Plasmid

#52372

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutant

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

deleted amino acids 1-70

Promoter

Ubiquitin

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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moPrP(3F4) D177N

Plasmid

#1319

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP D177N (Prnp Mouse)

Expression

Mammalian

Mutation

D177N point mutation

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pNY-347

Plasmid

#231047

Purpose

Used for GFP staining

Depositor

Jeremy Nathans

Insert

anti GFP DARPin fused to mouse Fc

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-c-Fos [N486/76R]

Plasmid

#177560

Purpose

Mammalian Expression Plasmid of anti-c-Fos (Human). Derived from hybridoma N486/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-c-Fos (Homo sapiens) recombinant mouse monoclonal antibody (FOS Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-4

Plasmid

#11363

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-4 (Irs4 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

3F4 epitag

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
iSH-iRFP670-sspB

Plasmid

#176102

Purpose

Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

phosphatidylinositol 3-kinase (Pik3r1 Mouse)

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pINTO-NFH::mEzh2

Plasmid

#65925

Purpose

Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

Enhancer of zeste homolog 2 (Ezh2 Mouse)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV/TO (T-REx) and CMV/TetO2 (T-REx)

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPm-DAD

Plasmid

#25410

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

mDia2 Amino Acids 1031-1171; Q1091P mutation

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR2-Fv-Fvls-E

Plasmid

#15286

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR2 cytoplasmic domain FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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Oct4-ires-EGFP(lox neo)

Plasmid

#21547

Depositor

Rudolf Jaenisch

Article

Lengner et al Cell Stem Cell. 2007 Oct 11;1(4):403-15.

Insert

EGFP, Oct4

Use

Mouse Targeting

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Vglut2 in situ probe

Plasmid

#45639

Depositor

Jeffrey Macklis

Article

Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

Insert

Vglut2 in situ probe (Slc17a6 Mouse)

Use

In situ

Mutation

fragment contains nt 2477-2993 of slc17a6 (Vglut2…

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pcmv3-STING-C1471S-FLAG

Plasmid

#198188

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C1471S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 147 mutates to …

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31-PGK-GFP-Blast

Plasmid

#31515

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
mEmerald-H3.3-N-14

Plasmid

#54116

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

H3.3 (H3f3b Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits