176,017 results
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Plasmid#105576PurposeT7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusionDepositorInsertHuman IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
UseIn vitro transcriptionAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)
Plasmid#103864PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorPublicationInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTH100
Plasmid#84458PurposepJB38-NWMN29-30 + SarA_P1-sGFP-TermDepositorInsertSarA_P1-sGFP from pCM29
Tags6xHis (on sfGFP)Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-hSynapsin-Cre-WPRE
Plasmid#86641PurposeExpresses Cre in mammalian cellsDepositorInsertCre
UseCre/Lox, Lentiviral, Mouse Targeting, and Synthet…ExpressionMammalianPromoterhSynapsinAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorInsertMeasles virus H glycoprotein
ExpressionMammalianPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2C-3XtbGFP-PEST
Plasmid#69072PurposeExpresses 3XturboGFP-NLS-PEST under MERVL_LTRDepositorInsertturboGFP
ExpressionMammalianAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-AtU6-1
Plasmid#66202Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
TagsNLS from SV40 T antigen and mCherryExpressionMammalianPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas serviceAAV Retrograde, AAV2, AAV5, and AAV8InsertEGFP
UseAAVPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTurquoise2-pBAD
Plasmid#54844PurposeLocalization: Protein Expression Vector , Excitation: 434, Emission: 474DepositorTypeEmpty backboneTags6x His, T7 tag, and TEV siteExpressionBacterialPromoteraraBADAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
EGFP-IC2-FL
Plasmid#51409Purposeexpression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cellsDepositorAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1 human cofilin S3A
Plasmid#50860Purposeexpresses constituvely active cofilin fused to EGFP in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
TagsEGFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-puro-LacZ
Plasmid#39477DepositorInsertLacZ
UseLentiviralTags6xHis and V5ExpressionMammalianPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET MBP His6 LIC cloning vector (2Cc-T)
Plasmid#37237DepositorTypeEmpty backboneTagsHis6 and MBPExpressionBacterialPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmEGFP-1
Plasmid#36409DepositorTypeEmpty backboneUseFluorescent protein cassette for sub-cloningTagsmEGFPPromoterno promoterAvailable sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by