We narrowed to 18,870 results for: IRE
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Plasmid#128709PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5617
Plasmid#128706PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopB1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNLuc
Plasmid#118058PurposeGBPart - NLuc Luciferase CDSDepositorInsertNano Luciferase
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTHFD1L_1
Plasmid#106316PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Krox20 promoter pGL3-TATA
Plasmid#21260DepositorInsertKrox20 promoter (Egr2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCPP5588
Plasmid#128708PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopD1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5053
Plasmid#128707PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5530
Plasmid#128705PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPP3417
Plasmid#128724PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopY1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FOXO3 6A
Plasmid#24382DepositorInsertFOXO3 (FOXO3 Human)
UseTagsFLAGExpressionMammalianMutationT179A, S399A, S413A, S555A, S588A, and S626APromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-sh-mFAK C
Plasmid#37015DepositorInsertsh-mFAK C (Ptk2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterhU6Available sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-TCAB1
Plasmid#51630PurposeMammalian expression of 3xFLAG tagged TCAB1, N-terminal.DepositorInsertTCAB1 (WRAP53 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
UseTagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …PromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag6-iCAM-1
Plasmid#205210PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6DepositorInsertsynCAM iCAM1 and Lag6 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag18-iCAM-1
Plasmid#205207PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18DepositorInsertsynCAM iCAM1 and Lag18 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag42-iCAM-1
Plasmid#205205PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42DepositorInsertsynCAM iCAM1 and Lag42 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
UseTagsExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372APromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …PromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CIBN-CAAX
Plasmid#188989PurposeA lentiviral plasmid encoding plasma membrane-tagged CIBNDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsCAAXExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-AsCas12a_crRNA-site4 (MSP3511)
Plasmid#160139PurposeT7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4DepositorInsertAsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
UseIn vitro transcription of sgrna from t7 promoterTagsExpressionMutationPromoterT7Available sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL K-ras 3'UTR
Plasmid#14804DepositorInsertK-ras 3'UTR (Kras Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Pou4f3
Plasmid#158530PurposeExpresses human Pou4f3 in mammalian cellsDepositorInsertPOU Class 4 Homeobox 3 (POU4F3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_T
Plasmid#147556PurposeMammalian Expression of HsSmg7DepositorInsertHsSmg7 (SMG7 Human)
UseTagsExpressionMammalianMutationtwo silent mutation G216A, A1521G and one non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag-Pcbp2
Plasmid#184028PurposeCodon optimized Pcbp2 clone with N-terminal flag tagDepositorInsertPcbp2 (Pcbp2 Mouse)
UseTagsFlagExpressionMammalianMutationCodon optimizedPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRG36-ChAT
Plasmid#99069PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea ChAT (choline acetyltransferase)DepositorInsertChAT (choline acetyltransferase) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EAAARx8-CaaX
Plasmid#155224PurposeMPAct with increased search radiusDepositorInsertF-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-Luc
Plasmid#122694PurposeOutput expression of firefly luciferase under the activation of BenR transcription factor for cell-free expressionDepositorInsertFirefly luciferase
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only