We narrowed to 59,445 results for: cloning

1. p667-UBC-TLK2-V5-TurboID_IDG-K

   Plasmid

   #135250

   Purpose

   Gateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TLK2-V5-TurboID (TLK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-DEST-Strep2

   Plasmid

   #119753

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmU1-CasRx-non-targeting (pKW83)

   Plasmid

   #229051

   Purpose

   Cloning backbone for CasRx crRNA (Mouse U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTHSSe_52

   Plasmid

   #109250

   Purpose

   ColE1 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. P-BT3332

   Plasmid

   #188110

   Purpose

   pBolux harboring the promoter region upstream of BT3332

   Depositor

   Guy Townsend

   Article

   Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

   Insert

   The B. thetaiotaomicron BT3332 promoter cloned into pBolux
   

   Expression

   Bacterial

   Promoter

   BT3332

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1a-MTS-jAspSnFR3

   Plasmid

   #203461

   Purpose

   Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3
   

   Use

   Gateway entry vector

   Tags

   Mitochondrial localization signal

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnlacZ-ACN

   Plasmid

   #20304

   Depositor

   Mario Capecchi

   Article

   Wu et al Nat Protoc. 2008 . 3(6):1056-76.

   Insert

   nlacZ-ACN
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFTP1

   Plasmid

   #64952

   Purpose

   source of TpR FRT cassette

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   FRT-dhfrII-FRT
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD378

   Plasmid

   #91827

   Purpose

   Codon-optimized HaloTag donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   HaloTag-C1
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p{ie1-DsRed::GW,attB}

    Plasmid

    #182173

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Expression

    Insect

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p{ie1-eGFP::GW,attB}

    Plasmid

    #182172

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    eGFP
    

    Expression

    Insect

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4092677

    Plasmid

    #153883

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    wt;handful of SNPs as watermarks

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pITC

    Plasmid

    #192856

    Purpose

    Cloning and expression of transcription factor gene under control of naringenin inducible promoter

    Depositor

    Eveline Peeters

    Article

    Bernauw et al Methods Mol Biol. 2022;2516:113-141. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PfdeAR

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS050

    Plasmid

    #91826

    Purpose

    Codon-optimized mScarlet-I donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    mScarlet-I-C1
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTHSSe_53

    Plasmid

    #109251

    Purpose

    pUC BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HUWE1

    Plasmid

    #25211

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    huwe1.4005.4374 (HUWE1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    HECT domain

    Available Since

    Feb. 18, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pTHSSe_51

    Plasmid

    #109249

    Purpose

    p15A BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p667-UBC-PRKCQ-V5-miniTurbo_IDG-K

    Plasmid

    #170590

    Purpose

    Gateway destination clone of PRKCQ (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    PRKCQ-V5-miniTurbo (PRKCQ Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMU102

    Plasmid

    #61805

    Purpose

    Cloning and expression vector for Clostridium thermocellum

    Depositor

    Lee Lynd

    Article

    Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK-Prrn (N. tabacum)

    Plasmid

    #123490

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    Prrn (N. tabacum)
    

    Use

    Synthetic Biology

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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