We narrowed to 9,260 results for: Coli
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Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCD442
Plasmid#153024PurposedCas9, MCP-SoxS(R93A/S101A)DepositorInsertdCas9, MCP-SoxS(R93A/S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_AB
Plasmid#66004PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLY152
Plasmid#130950Purposedcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)DepositorInsertsdcas9
tetR
sfgfp
UseSynthetic BiologyTagsASV tagExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPpspA-2G6 and PtetAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAT-9208
Plasmid#124221PurposeCloning plasmid for a self-targeting gRNA libraryDepositorInsertself-targeting gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPA-fapO-RgTALsyn
Plasmid#100946PurposeExpression of Tyrosine Ammonia Lyase from constitutive GAP promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterGAP (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-mScarlet-T7
Plasmid#173227PurposeEncodes mScarlet fluorescent protein. To be used in cell-free systemDepositorInsertmScarlet
UseCell-free systemTagsExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEST-p70a-UTR3-deGFP-6xHis-T500
Plasmid#92224PurposeExpression of deGFP using the p70a promoter and UTR3DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP6
Plasmid#71469PurposeEncodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 6 (SENP6 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
JBEI-3716
Plasmid#100169PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertcodon-optimized MK
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p148
Plasmid#62616PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
y2
Plasmid#53586PurposeProduces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2353_Tier3(PB)-YPet_PuroR
Plasmid#169652PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LacI/109-pHG165c
Plasmid#90058PurposeWT LacI polymorphism with T at position 109DepositorInsertLacI/109
UseTagsExpressionBacterialMutation109PromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only