We narrowed to 18,336 results for: met

1. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-ChrimsonR-GFP

   Plasmid

   #58806

   Purpose

   AAV expression of ChrimsonR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   Syn

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

   Plasmid

   #104567

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
   

   Use

   CRISPR

   Tags

   NLS-3xHA-VPR

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GalT-RpHLuorin2

   Plasmid

   #171719

   Purpose

   Trans-Golgi expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR I-Ab beta Derp1-117

   Plasmid

   #113639

   Purpose

   Insect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptide

   Depositor

   James Moon

   Article

   Moon et al Methods Mol Biol. 2018;1799:165-181. doi

   Insert

   I-Ab beta Derp1-117 (H2-Ab1 Mouse)
   

   Use

   Tags

   6x His tag, fused to Der p 1 (117-127) peptide vi…

   Expression

   Insect

   Mutation

   transmembrane and cytoplasmic domains truncated

   Promoter

   Metallothionein

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DRH337: AAV-hsyn-CheRiff-eGFP

   Plasmid

   #51697

   Purpose

   AAV production of CheRiff channelrhodopsin actuator

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   CheRiff-eGFP
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   hSynapsin

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

   Plasmid

   #55639

   Purpose

   Flp-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC476 - pAAV c-fos eYFP

    Plasmid

    #47907

    Purpose

    An AAV vector that expresses eYFP under the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    c-fos

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV_NLS-dSaCas9-NLS-VPR

    Plasmid

    #68495

    Purpose

    AAV vector containing nuclease null SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    dSaCas9
    

    Use

    AAV

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule2 - Mj Acd A9

    Plasmid

    #197652

    Purpose

    E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

    Depositor

    Ryan Mehl

    Article

    Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

    Inserts

    Mj Acd A9 synthetase
    Methanocaldococcus jannaschii tRNA
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32A, L65D, F108L, Q109E, D158S, L162T

    Promoter

    GlnS and lpp

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-miniDi-P2A-mCherry

    Plasmid

    #204358

    Purpose

    AAV vector for miniDi expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDi-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM4Di was …

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

    Plasmid

    #182423

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-eythropoietin (EPO Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-miniDq-P2A-mCherry

    Plasmid

    #204357

    Purpose

    AAV vector for miniDq expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM3Dq was …

    Promoter

    hSyn

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-G6-vioABE

    Plasmid

    #73438

    Purpose

    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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