2,226 results

1. pBa.Kif5c 1-559.GFP

   Plasmid

   #45059

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

   Insert

   Kif5c (Kif5c Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   motor domain aa1-559

   Promoter

   Chicken Beta Actin

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV302

   Plasmid

   #119943

   Purpose

   Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

   Insert

   N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218863

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCpf1-eBE

   Plasmid

   #107688

   Purpose

   Expresses dLbCpf1-eBE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 barr1 HA

   Plasmid

   #14693

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   beta arrestin 1 (Arrb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-HF-BE3

   Plasmid

   #87439

   Purpose

   Expresses HF-BE3 with C-terminal NLS in mammalian cells

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

   Insert

   HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mammalian codon-optimized Cas9-HF

   Promoter

   CMV

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BE-VQRCas9

   Plasmid

   #101740

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, VQR mutations

   Promoter

   CMV

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-GCaMP3bright

   Plasmid

   #74423

   Purpose

   Express fluorescent calcium indicator GCaMP3bright in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3bright
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D392A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-rSyntaxin-3a

   Plasmid

   #195206

   Purpose

   Mammalian expression vector for GFP tagged Syntaxin-3a.

   Depositor

   David Bryant, Thomas Weimbs

   Article

   Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

   Insert

   STX3A (Stx3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFSW GAP43-jRGECO1a P2A Syp-Halo

    Plasmid

    #185565

    Purpose

    Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

    Insert

    GAP43-jRGECO1a P2A Syp-Halo
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ALK7 K222R

    Plasmid

    #80887

    Purpose

    mammalian expression of ALK7 K222R

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K222R

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA TrkC

    Plasmid

    #24089

    Depositor

    Moses Chao

    Article

    Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

    Insert

    TrkC (Ntrk3 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG.PKCzeta.T410A

    Plasmid

    #10801

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Activation loop inactive: T410A (Thr 410 to Ala)

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-rSyntenin1

    Plasmid

    #89431

    Purpose

    mammalian expressio of EGFP tagged rat Syntenin1

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-mEGFP-CREB (S133A)

    Plasmid

    #137002

    Purpose

    mEGFP-CREB donor for G-CREB with S133A mutation

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Changes Serine 133 into Alanine

    Promoter

    CMV

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-GCaMP6-210

    Plasmid

    #91778

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-210
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI-Neo-HKIImut

    Plasmid

    #99240

    Purpose

    Type II Hexokinase expressed in AS-30D rat hepatoma cell line

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

    Insert

    Hexokinase II (Hk2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Four silent mutations; P (115)L, A (192) V, F (78…

    Promoter

    CMV I.E.

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV ORANGE Gria1-HaloTag KI

    Plasmid

    #139655

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Ef1a-DIO-OMOR-eYFP

    Plasmid

    #66847

    Purpose

    Expresses Opto-MOR in a Cre dependent manner

    Depositor

    Michael Bruchas

    Article

    Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

    Insert

    Opto Mu opioid receptor
    

    Use

    AAV and Cre/Lox

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid

    #123615

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A/K34A)-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A/K34A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. cacna2d1 HA pCAGGS

    Plasmid

    #206107

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

    Depositor

    Annette Dolphin

    Article

    Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-MyosinVb-Ctail

    Plasmid

    #110170

    Purpose

    Dominant negative effect by competing with MyosinVb C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1262-1670

    Promoter

    CMV

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFSynW Syt1 I367T IRES mRuby3

    Plasmid

    #170642

    Purpose

    Expression of syt1 in cultured neurons by lentiviral transduction

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    I367T

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSV Sport SREBP-1c dom neg

    Plasmid

    #8885

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

    Insert

    SREBP-1c (Srebf1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y305A amino acid substitution in the active versi…

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YFP-DBD

    Plasmid

    #14874

    Depositor

    Alexandra Newton

    Article

    Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

    Insert

    DAG binding domain (Prkcb Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Y123W

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pMT2 Beta1b GFP

    Plasmid

    #89893

    Purpose

    Mammalian expression of Beta1b GFP

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP sequence inserted just before the Stop codon

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pORANGE GFP-Bsn KI

    Plasmid

    #139664

    Purpose

    Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMT2 Beta1b mCherry

    Plasmid

    #89892

    Purpose

    Mammalian expression of Beta1b mCherry

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry sequence inserted just before the Stop co…

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge