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Showing: 1051 - 1100 of 11379 results

  4. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105635-plasmid-map-sequence-id-203205

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  5. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105637-plasmid-map-sequence-id-203819

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  6. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

    Plasmid
    #105638
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Champ1 (CHAMP1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105638-plasmid-map-sequence-id-203917

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  21. pTYB-pLAC-F112S

    Plasmid
    #48727
    Purpose
    Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine
    Depositor
    Gordon Laurie
    Article
    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6
    Insert
    Lacritin (LACRT Human)
    Use
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    Phenylalanine 12 to Serine
    Promoter
    T7
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. T4 Lysozyme K83H A112D WT*

    Plasmid
    #18399
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K83H A112D WT*
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  26. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  32. R777-E112 Hs.IRS2-nostop

    Plasmid
    #70396
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70396-plasmid-map-sequence-id-125845

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  35. pLenti X2 Blast/shp16 (w112-1)

    Plasmid
    #22261
    Depositor
    Eric Campeau
    Insert
    sh cyclin-dependent kinase inhibitor 2A
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2009
    Availability
    Academic Institutions and Nonprofits only
    22261-plasmid-map-sequence-id-10201

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  40. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  47. pPICZ:K2P2.1(mTREK-1)Cryst S112C

    Plasmid
    #224780
    Purpose
    Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter
    Depositor
    Dan Minor
    Article
    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1
    Insert
    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …
    Promoter
    AOX1 promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224780-plasmid-map-sequence-id-445194

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Showing: 1051 - 1100 of 11379 results