We narrowed to 4,416 results for: Pet2
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Plasmid#162559PurposeExpresses C. elegans iPGM containing a C-terminal biotinylation site and His tagDepositorInsertC. elegans iPGM bio-6His (ipgm-1 Nematode)
Tags6His tag, T7 tag, Thrombin cleavage site, and bio…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Bm-iPGM-bio
Plasmid#162565PurposeExpresses B. malayi iPGM containing a C-terminal biotinylation site and His tagDepositorInsertB. malayi iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-NLS-M-MLV-RT
Plasmid#181804PurposepET28a-NLS-M-MLV RTDepositorInsertNLS-M-MLV-RT
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL18
Plasmid#183389PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialPromoterlacAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-miniE-DBCO
Plasmid#181985PurposeUsed to label proteins with the MiniR genetically encoded tagDepositorInsertMiniE-DBCO
TagsDBCO-Tag, His-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
Tags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-4
Plasmid#228986PurposeFor bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-4
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-2
Plasmid#228996PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaGIgG-12
Plasmid#228999PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaGIgG-12
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
TagsHis6, LgBiTExpressionBacterialAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIV p24
Plasmid#212195PurposeBacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminusDepositorInsertChain A, Capsid protein p24, HIV type 1
TagsPolyhistidineExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b
Plasmid#122850PurposeExpresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coliDepositorInsertm(KS_MAT)_mouseL_ACP (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialPromoterT7Available SinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Tipin-His
Plasmid#23122DepositorAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpyCatcher002-MBP
Plasmid#216295PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cellsDepositorInsertSpyCatcher002-MBP
TagsHis6 tag and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f
Plasmid#171612PurposeAsCas12f1 heterologous expression plasmidDepositorInsertAsCas12f1
ExpressionBacterialAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-C2m2-mKate
Plasmid#208765PurposeTo express C2m2-mKate in bacteriaDepositorInsertC2m2-mKate (Mfge8 Mouse)
TagsmKateExpressionBacterialMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterT7 promoterAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-His6
Plasmid#51532PurposeExpress His6-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Mff(1-61)-PP-GST
Plasmid#73042PurposeBacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage siteDepositorInsertMff(1-61) (Mff Mouse)
TagsGST-tag and PreScission protease siteExpressionBacterialMutationTruncation containing amino acids 1-61PromoterT7Available SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
VV001: WT Arch-3 in pET28b
Plasmid#58487PurposeExpresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3 (with C-terminal His tag)
ExpressionBacterialPromoterT7Available SinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-GST
Plasmid#51528PurposeExpress GST-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-trAtCCD8(pYL735)
Plasmid#178286PurposeExpresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
ExpressionBacterialPromoterT7Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-10
Plasmid#172743PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-10
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM34
Plasmid#89156PurposeRecombinant expression of PTBP1-RRM34 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationcontains RNA Recognition Motif 3+4PromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only