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We narrowed to 4,300 results for: Pet2

Showing: 1051 - 1100 of 4300 results

  2. pET28a-RBP-LOV2

    Plasmid
    #185792
    Purpose
    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-LOV2
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185792-plasmid-map-sequence-id-367546

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  3. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Article
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71827-plasmid-map-sequence-id-254300

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  5. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  9. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  14. pET28a-HaloTag7SS-DogTag

    Plasmid
    #171775
    Purpose
    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    HaloTag7SS-DogTag
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    DogTag inserted in HaloTag7 between residues D139…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171775-plasmid-map-sequence-id-338524

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  15. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  23. pET28-Mff(1-61)-PP-GST

    Plasmid
    #73042
    Purpose
    Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Mff(1-61) (Mff Mouse)
    Use
    Tags
    GST-tag and PreScission protease site
    Expression
    Bacterial
    Mutation
    Truncation containing amino acids 1-61
    Promoter
    T7
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73042-plasmid-map-sequence-id-134011

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  38. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109419-plasmid-map-sequence-id-211590

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  39. pET21-pelB-LaG-6

    Plasmid
    #172741
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-6
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172741-plasmid-map-sequence-id-340175

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  48. pET28-iLOV-Rsn2

    Plasmid
    #61687
    Purpose
    bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV
    Depositor
    Brian Smith
    Insert
    Ranaspumin-2
    Use
    Tags
    His and iLOV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61687-plasmid-map-sequence-id-259998

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Showing: 1051 - 1100 of 4300 results