We narrowed to 128,198 results for: CIL

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lenti sgRNA(MS2)_puro backbone

Plasmid

#73795

Purpose

3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

IRES-EGFP

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

95 Articles

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EZ-Tet-pLKO-Blast

Plasmid

#85973

Purpose

Tet/Dox inducible shRNA lentivirus with blasticidin selection

Depositor

Cindy Miranti

Publication

Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

Type

Empty backbone

Use

Lentiviral and RNAi

Promoter

H1 / TetO

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

134 Articles

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pLenti CMV/TO SV40 small + Large T (w612-1)

Plasmid

#22298

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

44 Articles

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Publication

Scaffidi et al Nat Cell Biol. 2008 Apr;10(4):452-9. doi

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pLV-mitoGFP

Plasmid

#44385

Depositor

Pantelis Tsoulfas

Publication

Kitay et al Hum Mol Genet. 2013 Jan 24.

Insert

mitoGFP (COX8A Human)

Use

Lentiviral

Tags

Cox8 targeting sequence and GFP

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Publication

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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Tet-O-FUW-Ascl1

Plasmid

#27150

Depositor

Marius Wernig

Publication

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pMK243 (Tet-OsTIR1-PURO)

Plasmid

#72835

Purpose

Expresses OsTIR1 under the control of the Tet promoter

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-Tet-OsTIR1-PURO-AAVS1

Use

CRISPR

Expression

Mammalian

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Publication

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Publication

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Publication

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Publication

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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p414-TEF1p-Cas9-CYC1t

Plasmid

#43802

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

Depositor

George Church

Publication

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

TEF1 promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

70 Articles

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p426-SNR52p-gRNA.CAN1.Y-SUP4t

Plasmid

#43803

Purpose

Encodes a gRNA that targets Can1.Y in yeast.

Depositor

George Church

Publication

Dicarlo et al Nucleic Acids Res

Insert

CAN1.y gRNA (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

61 Articles

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Publication

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pLX301

Plasmid

#25895

Purpose

3rd generation lentiviral Gateway destination vector. Puromycin selection.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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