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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 70,757 results for: LAS

Showing: 6571 - 6600 of 70757 results

  2. pMP597

    Plasmid
    #134999
    Purpose
    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134999-plasmid-map-sequence-id-267926

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  7. RH5-bio

    Plasmid
    #47780
    Purpose
    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised RH5
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47780-plasmid-map-sequence-id-68799

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  10. pUC19-TSV (linearized in LT)

    Plasmid
    #70033
    Purpose
    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSPyV full genome, linearized in Large T-antigen
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70033-plasmid-map-sequence-id-129217

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  13. pAM4788

    Plasmid
    #120089
    Purpose
    Cyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.
    Depositor
    Susan Golden
    Article
    Chen et al Microbiology. 2016 Dec;162(12):2029-2041
    Insert
    pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120089-plasmid-map-sequence-id-234417

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  20. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65779-plasmid-map-sequence-id-112660

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  21. MSP3-bio

    Plasmid
    #47731
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP3
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47731-plasmid-map-sequence-id-68466

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  22. MSP2-bio

    Plasmid
    #47710
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP2
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47710-plasmid-map-sequence-id-68424

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  23. AA406

    Plasmid
    #216070
    Purpose
    Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216070-plasmid-map-sequence-id-431465

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  24. LLP790_αGCN4-EZH2-FL-CO

    Plasmid
    #211784
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-KRAB
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect KRAB e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211784-plasmid-map-sequence-id-417361

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  30. MSP7-bio

    Plasmid
    #47735
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP7
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47735-plasmid-map-sequence-id-68747

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Showing: 6571 - 6600 of 70757 results