We narrowed to 8,811 results for: CAG

1. pMK312 (TOP2A CRISPR)

   Plasmid

   #140654

   Purpose

   TOP2A tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-mCherry-SPASTIN

   Plasmid

   #180647

   Purpose

   Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Starts at M87, has siRNA resistance to GAACAGUGUG…

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

4. PX458_AHR_1

   Plasmid

   #101076

   Purpose

   Encodes gRNA for 3' target of human AHR

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against AHR (AHR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cytosolic Fyn FRET Biosensor

   Plasmid

   #124654

   Purpose

   FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO_EGFP-RandE3

   Plasmid

   #79872

   Purpose

   For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC91-pmax-dCas9VP64

   Plasmid

   #48223

   Purpose

   dCas9VP64 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP64 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_KDM1A_1

   Plasmid

   #86297

   Purpose

   Encodes gRNA for 3' target of human KDM1A

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KDM1A (KDM1A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_KDM1A_2

    Plasmid

    #86298

    Purpose

    Encodes gRNA for 3' target of human KDM1A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM1A (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pv6_MBD1_MBD

    Plasmid

    #179404

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLL5.0 hCortKD EGFP

    Plasmid

    #187267

    Purpose

    Lentiviral expression of shRNA targeting Cortactin

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    shRNA for Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL.175

    Plasmid

    #184996

    Purpose

    Express -Eco1 HEK3 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK3 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL.177

    Plasmid

    #184998

    Purpose

    Express -Eco1 EMX1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    EMX1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgMLST8_1

    Plasmid

    #124905

    Purpose

    Targeting human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmIL-6 mut C/EBP

    Plasmid

    #61291

    Purpose

    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated C/EBP binding site from ACATTGTGCAATCT to…

    Promoter

    IL-6 promoter with mutant C/EBP binding site

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. lenti-GBA-N370S-tmpknot-epegRNA

    Plasmid

    #214091

    Purpose

    Lentiviral vector expressing epegRNA to induce GBA N370S mutation

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    GBA N370S epegRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PX458_AHR_2

    Plasmid

    #101077

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEBTet-SNAP-CCCAP

    Plasmid

    #136798

    Purpose

    Mammalian expression of the centrosomal protein CCCAP N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-CCCAP (SDCCAG8 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. LentiCRISPR v2 mBAK

    Plasmid

    #129578

    Purpose

    CRISPR deletion of murine BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAK (Bak1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px458_2A_GFP_sgRNA_G3BP2_G1

    Plasmid

    #127112

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA G3BP2 (G3BP2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LENTICRISPR-PGAM1_sgRNA1

    Plasmid

    #201614

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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