We narrowed to 9,202 results for: sacs

1. POT5-RFP

   Plasmid

   #65321

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS414-URR

   Plasmid

   #65329

   Purpose

   CEN plasmid as receiving vector for exogenous pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR1-RFP-Leu2-URR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMV-URR2

   Plasmid

   #65332

   Purpose

   standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. POT3-RFP

   Plasmid

   #65319

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. POT9-RFP

   Plasmid

   #65325

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMV-URR1

   Plasmid

   #65331

   Purpose

   standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46593

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-16 Decoy (Mir16-1 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pILGFP86

   Plasmid

   #83543

   Purpose

   Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   RPL4A promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPL4A

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc-HT-Dmp53

   Plasmid

   #85698

   Purpose

   expression of Drosophila p53 in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   Dmp53 (p53 Fly)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Ac5

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pCfB2800

    Plasmid

    #63657

    Purpose

    pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBacPAK8-His-myc-Uba1

    Plasmid

    #29508

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Uba1 (UBA1 Budding Yeast)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CMVpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ERKsubDD-Venus-NES

    Plasmid

    #84630

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ERKsubDD-Venus-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ptagBFP-TetR-NLS-VP16

    Plasmid

    #103835

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BAS289 C terminal E2C G418R

    Plasmid

    #74084

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pU6Rosa-CAG-Gal4-Rad18UBD

    Plasmid

    #119012

    Purpose

    Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Gal4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA3

    Plasmid

    #46790

    Purpose

    To express ATPase inactive Fun30 in budding yeast

    Depositor

    Patrick Varga-Weisz

    Article

    Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

    Insert

    FUN30 (FUN30 Budding Yeast)
    

    Use

    Tags

    V5

    Expression

    Yeast

    Mutation

    ATP-binding site mutated replaced lysin 603 by ar…

    Promoter

    PGAL1

    Available since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221-RAP1

    Plasmid

    #84525

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    none

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNM3746

    Plasmid

    #154109

    Purpose

    loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::His-58::FRT3
    mex-5p::FLP D5::sl2::mNG::glh2 3'
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2799

    Plasmid

    #63658

    Purpose

    pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSA1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB2992

    Plasmid

    #63642

    Purpose

    pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-RLuc8C-FHA1-NES

    Plasmid

    #138210

    Purpose

    Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    RLuc8C-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEPOR1CB0113

    Plasmid

    #117552

    Purpose

    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTH734-CEN-minHIS3

    Plasmid

    #38227

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTH737-CEN-maxHIS3

    Plasmid

    #38229

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. A102 ΔAmpH Δ2x+ve

    Plasmid

    #129484

    Purpose

    Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKV005

    Plasmid

    #64763

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Use

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BAS291 C terminal mKO2 kanR

    Plasmid

    #74086

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only