We narrowed to 90,081 results for: Mal

Showing: 6631 - 6660 of 90081 results

Results per page:

Sort results by:

pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hNANOG (Plath)

Plasmid

#18115

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Nanog homeobox (NANOG Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2_hMGFP-60A

Plasmid

#200393

Purpose

A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

monster GFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-I-SceI-Trex2

Plasmid

#44024

Depositor

Jeremy Stark

Article

Gunn et al J Biol Chem. 2011 Dec 9;286(49):42470-82

Insert

I-SceI fused to mTREX2

Tags

NLS-HA

Expression

Mammalian

Promoter

pCAGGS

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Cas9-NRTH

Plasmid

#136925

Purpose

Expresses Cas9-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ GFP-2xESP3I-IRES-mCherry

Plasmid

#230996

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Type

Empty backbone

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FCK-Halo-GFP

Plasmid

#14750

Purpose

Lentiviral expression of Halo-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al PLoS One. 2007 Mar 21;2(3):e299.

Insert

Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N. pharaonis halorhodopsin is mammalian codon-opt…

Promoter

CaMKIIa

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Expression

Mammalian

Promoter

Tet-CMV

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Keima-cb5

Plasmid

#137755

Purpose

Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

Keima-cb5

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Tags

myc

Expression

Mammalian

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-PAT1

Plasmid

#88856

Purpose

myc-TAG overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

SLC36A1 (SLC36A1 Human)

Tags

myc

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK_hygro_BirA

Plasmid

#136537

Purpose

Lentiviral expression vector for an inducible BirA

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T3-hCAS-pA

Plasmid

#48625

Purpose

Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii, Kunihiko Naito

Article

Fujii et al Nucleic Acids Res. 2013 Aug 30.

Insert

Codon optimized Cas9

Use

CRISPR

Tags

FLAG, NLS, and polyA

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(GCC3)

Plasmid

#235078

Purpose

Expresses Tad-CBE(GCC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Tad-CBE(GCG4)

Plasmid

#235080

Purpose

Expresses Tad-CBE(GCG4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Ras-LOCKR-PL Key

Plasmid

#216503

Purpose

Ras activity dependent proximity labeler, Key only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Ras-LOCKR-PL Cage

Plasmid

#216502

Purpose

Ras activity dependent proximity labeler, Cage only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Cage

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-WIPI2d-TEVcs-TSF

Plasmid

#171420

Purpose

For the purifcation of GFP- WIPI2d protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

WIPI2d (WIPI2 Human)

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pClathrin-miRFP670nano

Plasmid

#127430

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-clathrin light chain

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PE2-VQR

Plasmid

#159982

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VQR

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pActin-miRFP670nano

Plasmid

#127428

Purpose

Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-human beta-actin

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

Plasmid

#34631

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

rat Syntaxin1a (Stx1a Rat)

Tags

meGFP

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0044-EF1a-RanCas13b-NES-mapk

Plasmid

#103855

Purpose

Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

RanCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and mapk NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_SARS-CoV-2_Sdel18

Plasmid

#207307

Purpose

Mammalian expression of SARS2 coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SARS-CoV2_S (S SARS2 coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge