We narrowed to 6,963 results for: RAP

1. pBK-VQR-BE3

   Plasmid

   #85171

   Purpose

   Expresses VQR-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VQR-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHPHS232

   Plasmid

   #228356

   Purpose

   Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Inserts

   mTagBFP2
   Reverse tetracycline transactivator 3
   neo
   

   Use

   Tags

   Blasticidin S deaminase and iCasp9

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and gene trap

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-sgAAVS1-HepmCherry

   Plasmid

   #192827

   Purpose

   Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRi

   Plasmid

   #199808

   Purpose

   vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

   Depositor

   Steve Salipante

   Article

   Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

   Inserts

   dCas9
   sgRNA with GFP cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-GFP-ATL2

   Plasmid

   #109020

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2 (ATL2 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRB_LD 0_CS G_tTA-BFP

   Plasmid

   #58876

   Purpose

   MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK88_GFP-Anillin

   Plasmid

   #46354

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   Anillin (ANLN Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   a1854 is changed to c to disrupt XhoI site withou…

   Promoter

   pBABE

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XFlagNICD4

   Plasmid

   #20186

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 4 Intracellular Domain (Notch4 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 4 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pBK-VRER-BE3

   Plasmid

   #85173

   Purpose

   Expresses VRER-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VRER-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBK-YEE-BE3

    Plasmid

    #85177

    Purpose

    Expresses YEE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FRB_nSuMMVp

    Plasmid

    #118970

    Purpose

    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSuMMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. N1-mSin1.2-GFP

    Plasmid

    #72908

    Purpose

    GFP-tagged mSin1 (isoform 2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. xCas9(3.6)-BE4

    Plasmid

    #108386

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. xCas9(3.6)-BE3

    Plasmid

    #108385

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. mEos2-CD9-10

    Plasmid

    #57356

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pBK-YE2-BE3

    Plasmid

    #85176

    Purpose

    Expresses YE2-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3 S6K2 E388 D3E

    Plasmid

    #17731

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

    Promoter

    CMV

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LBR-FKBP-GFP

    Plasmid

    #222413

    Purpose

    To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al bioRxiv 2024.05.31.596797

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

    Plasmid

    #170467

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

27. FRB_nSbMVp

    Plasmid

    #118969

    Purpose

    Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSbMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-eBE-S3

    Plasmid

    #101093

    Purpose

    Expresses eBE-S3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    eBE-S3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEL033

    Plasmid

    #137902

    Purpose

    STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJMP1363

    Plasmid

    #119279

    Purpose

    for stabilizing ICE in the presence of rapI expression

    Depositor

    Oren Rosenberg, Carol Gross, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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