We narrowed to 27,314 results for: sta

1. pCS2+ Lifeact-mCherry

   Plasmid

   #185408

   Purpose

   Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   lifeact
   

   Use

   In vitro synthesis of mrna

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px458_2A_GFP_sgRNA_RACK1

   Plasmid

   #127123

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA RACK1 (RACK1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV5 mSynCAM 2

   Plasmid

   #115497

   Purpose

   expression of mouse full-length SynCAM 2

   Depositor

   Thomas Biederer

   Article

   Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

   Insert

   Cadm2 (Cadm2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTSAR3.1t

   Plasmid

   #210254

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   mCerulean
   superfolder GFP
   

   Tags

   ssra

   Expression

   Bacterial

   Promoter

   ipaH7.8p and rpsM

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. UKNP1.21.5

   Plasmid

   #98203

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G-GECO1-Orai3

   Plasmid

   #73563

   Purpose

   Green fluorescent reporter of Orai3-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1-Orai3 (ORAI3 Human, Synthetic)
   

   Tags

   G-GECO1

   Expression

   Mammalian

   Promoter

   CMV Immdeiate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG2IG-Hmga2

   Plasmid

   #122294

   Purpose

   Overexpression for mouse Hmga2

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-OSF-VPS15

   Plasmid

   #99326

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS15 (PIK3R4 Human)
   

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pKanCMV-mRuby3-18aa-Tubulin

   Plasmid

   #74256

   Purpose

   Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   Tubulin
   

   Tags

   mRuby3

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGCP123-GFP_g1

    Plasmid

    #153518

    Purpose

    sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-mCherry2-mito

    Plasmid

    #164164

    Purpose

    Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2-mtActA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2-BiNLS-fYap

    Plasmid

    #176623

    Purpose

    expresses optofYap in zebrafish

    Depositor

    Timothy Saunders

    Article

    Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

    Insert

    Yes associated protein (yap1 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Promoter

    SP6

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCEP4-BG505.SOSIP-gp160

    Plasmid

    #123273

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIRECT_21A

    Plasmid

    #91128

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWzl_CRKL-Y207F+W275L

    Plasmid

    #196022

    Purpose

    Expresses CRKL Y207F + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWzl_CRKL-W275L

    Plasmid

    #196019

    Purpose

    Expresses CRKL W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UKNP1.23.1

    Plasmid

    #98206

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRedCm

    Plasmid

    #176225

    Purpose

    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PrhaB and PL

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. UKNP1.20.1

    Plasmid

    #98195

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-Myc CDC4 R465C

    Plasmid

    #16653

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    CDC4 R465C (FBXW7 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R465C

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

23. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    mRuby3

    Expression

    Yeast

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UKNP1.17.1

    Plasmid

    #98189

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. UKNP1.5.2

    Plasmid

    #97368

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. UKNP1.20.3

    Plasmid

    #98197

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Mutation

    codon optimized for human

    Promoter

    T7

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-miRE_shBRD9_561-SV40-GFP

    Plasmid

    #75139

    Purpose

    Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBRD9_561
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

30. mEos2-Mito-7

    Plasmid

    #57401

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Mito (COX8A Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

31. MSCV_JAK3_IRES_BFP

    Plasmid

    #206291

    Purpose

    Expresses the wild type form of human JAK3.

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1MycHis/HIP1

    Plasmid

    #29590

    Depositor

    Theodora Ross

    Article

    Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

    Insert

    Huntingtin-interacting protein 1 (HIP1 Human)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Available Since

    Nov. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-Dest47- C11orf83-GFP

    Plasmid

    #65844

    Purpose

    Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 WT (UQCC3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLKO mouse shRNA 1 raptor

    Plasmid

    #21339

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    raptor shRNA 1 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

36. His_Naa80d in pTYB12

    Plasmid

    #188455

    Purpose

    Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    Naa80 (NAA80 Human)
    

    Tags

    intein-CBD, 6xHis

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-CD4-FLAG

    Plasmid

    #51604

    Purpose

    expresses CD4 with C-terminal FLAG tag

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    CD4 (CD4 Human)
    

    Tags

    FLAG and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLAG HDAC5 WT

    Plasmid

    #32213

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pcFLAG RUNX3 (R122C)

    Plasmid

    #203438

    Purpose

    Mammalian expression of RUNX3

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    RUNX3 (RUNX3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCEP4-intron-BaLgp160

    Plasmid

    #100918

    Purpose

    Mammalian expression plasmid for Env from the BaL HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env with upstream intron
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKP3915

    Plasmid

    #113335

    Purpose

    Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Insert

    Artificial Transcription Factor (McIsaac et al, 2013)
    

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMXs-KDM4D

    Plasmid

    #188234

    Purpose

    Express human KDM4D in mammalian cells

    Depositor

    Young Tang

    Article

    Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

    Insert

    Lysine demethylase 4D (KDM4D Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Prolactin-CD4d3+4-bio

    Plasmid

    #73123

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    PRL (PRL Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRelA'

    Plasmid

    #175595

    Purpose

    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Catalytic domain of E.coli relA gene.
    

    Expression

    Bacterial

    Mutation

    Only the catalytic domain of the enzyme is used (…

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid

    #199251

    Purpose

    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SaCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CFAST-Halotag-ER

    Plasmid

    #214413

    Purpose

    ER localization of CFAST fused to Halotag-ER

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Tags

    CFAST-Halo

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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49. sIL6R PLAK1

    Plasmid

    #161519

    Purpose

    Expresses soluble IL6 receptor in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    soluble IL-6 receptor
    

    Use

    Lentiviral

    Tags

    3X Flag

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCas12a

    Plasmid

    #186446

    Purpose

    Encodes Cas12a from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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