We narrowed to 19,010 results for: Cre

1. pCMV_APOBEC1-UdgX-nCas9

   Plasmid

   #163531

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416 Gal G294A YFP

   Plasmid

   #27448

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Glycine 294 mutated to Alanine (G294A)

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

3. Sema6d.1-AP-His

   Plasmid

   #72042

   Purpose

   Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema6d.1 (Sema6d Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EF1a_NEUROG1_P2A_Hygro_Barcode

   Plasmid

   #120467

   Purpose

   Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROG1 (NEUROG1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KK501: pMVP (L3-L2) FKBP DD + polyA

   Plasmid

   #121789

   Purpose

   pMVP L3-L2 entry plasmid, contains FKBP DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   FKBP Degron Domain + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LB124 (Bcl-2 from -1287 to -3934)

   Plasmid

   #15240

   Depositor

   Linda Boxer

   Article

   Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial

   Mutation

   Bcl-2 fragment from -1287 to -3934 with P1

   Promoter

   Available since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pCMV7.1_3xFLAG-MOB1A

   Plasmid

   #172988

   Purpose

   constitutive expression of FLAG-tagged MOB1A

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-MOB1A (MOB1A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Sema4a-Fc-His

   Plasmid

   #72154

   Purpose

   Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4a (Sema4a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.10-VEGFprom(-950 to -700)

   Plasmid

   #66133

   Purpose

   Luciferase-based reporter for VEGF promoter region (-950 to -700 bp)

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   VEGF -950 to -700

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. DCT.VV.Orf37

    Plasmid

    #12725

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf37
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

11. DCT.VV.Orf48

    Plasmid

    #12736

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf48
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pECE-M2-PAK2 S20A

    Plasmid

    #31681

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 S20A (PAK2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 20 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCold TDP43 G294A

    Plasmid

    #27452

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

15. GFP-SP110C

    Plasmid

    #65393

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110C
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. DCT.VV.Orf40

    Plasmid

    #12728

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pInducer10-UBC9-sh4

    Plasmid

    #168988

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Syn-ChromeQ-GFP

    Plasmid

    #123317

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available since

    March 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a_POU1F1_P2A_Hygro_Barcode

    Plasmid

    #120473

    Purpose

    Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    POU1F1 (POU1F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-DNAJC5 (WT)-HaloTag

    Plasmid

    #205710

    Purpose

    Mammalian expression of DNAJC5 (WT)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA1 Flag ATXN1L

    Plasmid

    #33242

    Depositor

    Huda Zoghbi

    Article

    Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

24. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPDp

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ATF4 7: 5’3’ATF4.GFP

    Plasmid

    #21854

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Vector derived viral termination and poly-adenyla…

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Sema6d.1-Fc-His

    Plasmid

    #72168

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiCRISPRv2puro-Ptgs2_B6

    Plasmid

    #170300

    Purpose

    A knock-out vector for the mouse Ptgs2 in C57BL6

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

    Plasmid

    #192147

    Purpose

    T cell CRISPR lentiviral vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ntn4-Fc-His

    Plasmid

    #72106

    Purpose

    Expresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn4 (Ntn4 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAT9751-BEAR-mCherry-preedited

    Plasmid

    #162993

    Purpose

    BEAR control plasmid with split mCherry and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mCherry split with an intron between amino acids 119-120
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DCT.VV.Orf107

    Plasmid

    #12795

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf107
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

33. Arch(D95H)-eGFP

    Plasmid

    #51081

    Purpose

    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

    Depositor

    Adam Cohen

    Article

    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

    Insert

    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Histidine

    Promoter

    Ubiquitin

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LentiCRISPRv2puro-Tbxa2r

    Plasmid

    #170304

    Purpose

    A knock-out vector for the mouse Tbxa2r

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxa2r gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAC8_GWs-Lox (VE5589)

    Plasmid

    #163769

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    no insert
    

    Use

    Tags

    no tag

    Expression

    Insect

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCIG-Flag-Sox9-IRES-nls-GFP

    Plasmid

    #44281

    Depositor

    Martin Cheung

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

    Insert

    Flag-Sox9 (SOX9 Chicken)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE enhancer and Chicken beta-actin promoter

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pOCC175

    Plasmid

    #118890

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Sema3f(L,+)-AP-His

    Plasmid

    #72021

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOCC240

    Plasmid

    #118895

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease and mCherry, clea…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GST-BRD (1079-1198)

    Plasmid

    #99507

    Purpose

    Bacterial expression of mouse CBP bromodomain

    Depositor

    Peter Wright

    Article

    Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

    Insert

    mouse CBP BRD (1079-1198) (Crebbp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge