Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 30,195 results for: REP

Clear keywords
Showing: 4501 - 4520 of 30195 results

  8. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

    Enlarge

  9. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Depositor
    Geert van den Bogaart
    Article
    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pDEST-EGFP-Cx43ML-N1

    Plasmid
    #49860
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. No s…
    Promoter
    CMV
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. p3E DN Kif5a

    Plasmid
    #105973
    Purpose
    3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp
    Depositor
    Caren Norden
    Article
    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10
    Insert
    Kif5a (kif5aa Zebrafish)
    Expression
    Bacterial
    Mutation
    truncation of aa 416-1033
    Available Since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105973-plasmid-map-sequence-id-203068

    Enlarge

  20. pCAGGS_m-AKAR5’

    Plasmid
    #114140
    Purpose
    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5' C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114140-plasmid-map-sequence-id-215469

    Enlarge

Showing: 4501 - 4520 of 30195 results