171,784 results
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Plasmid#193846PurposeExpress TadDE (with SaCas9) in mammalian cellsDepositorInsertTadDE with SaCas9
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
JN302: pMVP/SB/Puro-DEST
Plasmid#121865PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…ExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD
Plasmid#169818PurposeExpresses C-terminal flag-tagged CAD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCCC -> AGTC silent mutations at nt527-530 eli…Available SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
Plasmid#208977PurposeEntry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)DepositorInsertpUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
UseCRISPRTagsBPNLSExpressionMammalianPromoterhuman U6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFN33K LgBiT TK-neo Human collagen type IV alpha 5 chain (COL4A5)
Plasmid#229768PurposeHuman Col4A5 tagged at N-terminal with LgBiT to be used with N-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay systemDepositorAvailable SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN-I-GFP
Plasmid#1736PurposeThis retroviral vector has an IRES-GFP downstream of the cloning sitesDepositorInsertIRES-GFP
UseRetroviralAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-C1
Plasmid#54801PurposeLocalization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneTagsmCherry-miniSOGExpressionMammalianAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt5B
Plasmid#35912DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEZT-BM
Plasmid#74099PurposeMulti-purpose rapid titer bacmam expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseBacmam, baculovirusTagsEGFP following p10 promoterExpressionMammalianPromoterCMV, p10Available SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-PinkyCaMP
Plasmid#232860PurposeAn AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoterDepositorInsertPinkyCaMP
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE
Plasmid#234895PurposeCAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tagDepositorInsertFRT(F3F5)-ChRmine3.3-p2A-oScarlet
UseAAVAvailable SinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pBT114
Plasmid#138523Purposephage cloningDepositorInsertphage genes
UseSynthetic BiologyAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTNS1
Plasmid#64967PurposeTn7 transposase expressionDepositorInserttnsABCD
Available SinceOct. 23, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLZ12Km2-P23R:TA:Xen5
Plasmid#88902PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + LuxABCDE
ExpressionBacterialAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
Tags6X-HisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR ARRET
Plasmid#111384PurposeARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertARRET
UseGateway entry cloningAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT27
Plasmid#223399PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.DepositorInsert2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CDK2 gRNA (BRDN0001148950)
Plasmid#77193Purpose3rd generation lentiviral gRNA plasmid targeting human CDK2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
POLN_pDEST17
Plasmid#64885Purposebacterial expression of POLN for purificationDepositorAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMH1742
Plasmid#160929PurposeExpression of soluble Sc octamer (Cse4)DepositorInsertsHHF1
HTA1
HTB1
Cse4
Tags6His-TEV, N-terminal 6His-TEV, and noneMutationcodon-optimized for E. coli -- see provided seque…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-CRAF(FL) BiBiT-Ready Vector
Plasmid#236799PurposeExpress LgBiT-CRAF(FL) Fusion Protein in Mammalian Cells under a CMV promoterDepositorAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB-CMV-MCS-Puro GEM-LTB4
Plasmid#202641PurposeExpresses the LTB4 sensor GEM-LTB4 in mammalian cellsDepositorInsertGEM-LTB4
ExpressionMammalianPromoterCMVAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only