162,717 results
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lentiCRISPR - EGFP sgRNA 1
Plasmid#51760PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)
Plasmid#214876PurposeLentiviral vector encoding RfxCas13d nontargeting control guideDepositorInserthU6-crNT-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)
Plasmid#213027PurposeExpresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationD702Y (RT- reverse transcriptase catalytic dead)PromoterCMVAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
trLATmEos3.2
Plasmid#203745PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrLAT (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
HDR-HaloPOLQ
Plasmid#211637Purposehalo donorDepositorInsertPuro-LoxHaloTag
UseTagsflagExpressionMammalianMutationPromoterSV40Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2056
Plasmid#142913PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX2-8 (NKX2-8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3282
Plasmid#144758PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBRCA1 (BRCA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF525-AcrIIA4_gb39_lenti
Plasmid#138305PurposeLenti expression of AcrIIA4DepositorInsertAcrIIA4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-R1
Plasmid#52638PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertRight AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 9b
Plasmid#134907PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.DepositorInsertMyo 9b (rat) with 3' UTR (Myo9b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZT-AAVS1-L1
Plasmid#52637PurposeTALEN-mediated gene editing at human PPP1R12C/AAVS1 locusDepositorInsertLeft AAVS1 TALEN
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic EKAR (Cerulean-Venus)
Plasmid#18679DepositorInsertERK Activity Reporter
UseTagsmonomeric Cerulean and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
APEX2-V5-G3BP1_pTRE-G418
Plasmid#202013Purposeexpresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertAPEX2-V5-G3BP1
UseLentiviralTagsExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP
Plasmid#102935PurposeAn AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoterDepositorInsertNK1R (Tacr1 Rat)
UseAAVTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt1
Plasmid#35905DepositorInsertWnt1 (WNT1 Human)
UseTagsExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 T56N
Plasmid#86789Purposemammalian expression of Rab12 T56N, constitutively GDP-bound mutantDepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationT56N, constitutively GDP-bound mutantPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NeonGreen-C1-ORP6
Plasmid#220072Purposeoverexpresses ORP6 with a neonGreen fluorophoreDepositorInsertOSBPL6 (OSBPL6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Egr1
Plasmid#11729DepositorInsertEgr-1 (Egr1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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huCaV2.2 (pSAD442-1)
Plasmid#62574PurposeHuman CaV2.2 expression in mammalian cellsDepositorInsertCACNA1B (CACNA1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ782 - HygroR
Plasmid#190933PurposeHygromycin resistance co-injection markerDepositorInsertHygromycin resistance gene
UseTagsExpressionWormMutationPromoterrps-0Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS31: pTwinStrep-SUMO-PmcTnsAB
Plasmid#168164PurposePmcTnsAB expression construct for protein purification.DepositorInsertPmcCAST TnsAB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Luc
Plasmid#45968DepositorInsertLuciferase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorInsertNEUROG2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLOW30
Plasmid#172697PurposeC. elegans mScarlet co-injection markerDepositorInsertPmyo-2::mScarlet
UseTagsmScarletExpressionWormMutationPromotermyo-2Available sinceSept. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits
