19,640 results

1. pcDNA3-mRuby2

   Plasmid

   #40260

   Purpose

   RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   mRuby2
   

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

   Plasmid

   #194458

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mH2A1.2-CT-MYC

   Plasmid

   #45168

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Tags

   HIS and MYC

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

6. Arf3-GFP

   Plasmid

   #39555

   Depositor

   Paul Melancon

   Article

   Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

   Insert

   ADP ribosylation factor 3 (ARF3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pAWP78

   Plasmid

   #61263

   Purpose

   Small IncP-based broad host range replicating vector.

   Depositor

   Mary Lidstrom

   Article

   Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

   Plasmid

   #166055

   Purpose

   Expresses the full length TurboID on the surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ104 - Pmyo-3::mCherry::unc-54

   Plasmid

   #19328

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-3::mCherry
   

   Tags

   mCherry

   Expression

   Worm

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPF185

    Plasmid

    #106367

    Purpose

    C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Mutation

    codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #125692

    Purpose

    Global labeling of inhibitory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo RYR-1

    Plasmid

    #75113

    Purpose

    Clone encoding rabbit ryanodine receptor in mammalian expression vector

    Depositor

    Paul Allen, Robert Dirksen

    Article

    Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

    Insert

    Ryanodine receptor 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MIB2-FLAG

    Plasmid

    #37117

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-EPHA2

    Plasmid

    #116732

    Purpose

    Lentiviral expression of EPHA2

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Myc-IQGAP1

    Plasmid

    #30118

    Depositor

    David Sacks

    Article

    Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV/TO eGFP Puro (w159-1)

    Plasmid

    #17481

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-mPIAS3

    Plasmid

    #15207

    Depositor

    Ke Shuai

    Article

    Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

    Insert

    PIAS3 (Pias3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V580I

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-ERBB3

    Plasmid

    #23874

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORI28

    Plasmid

    #71595

    Purpose

    EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

    Depositor

    Todd Klaenhammer, Jan Kok

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Type

    Empty backbone

    Use

    Integration vector for bacteria

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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