We narrowed to 39,129 results for: Eras

1. pKL1-424

   Plasmid

   #219412

   Purpose

   Reporter gene (3 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x3-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL1-095

   Plasmid

   #219407

   Purpose

   Reporter gene (4 operators) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (13-6 op)x4-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKL1-423

   Plasmid

   #219411

   Purpose

   Reporter gene (2 operator) for zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   (42-10 op)x2-minCyc1-Venus-tCyc1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ph-NylC_pET28a

   Plasmid

   #215425

   Purpose

   Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   Ph-NylC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Noodles Plasmid

   Plasmid

   #212613

   Purpose

   An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

   Depositor

   Ilya Vinnikov

   Article

   Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K

   Plasmid

   #214460

   Purpose

   Gateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CSNK1G3 (CSNK1G3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

   Plasmid

   #214458

   Purpose

   Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. β2AR-PPI-CALPon1.0 (APEX)

   Plasmid

   #213406

   Purpose

   Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ca-LiPPI-CLAPon1.0 (TEVp)

   Plasmid

   #213409

   Purpose

   Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   CaM-V5-TEVp
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pkk223_RbMutY_D146N_E45Q

    Plasmid

    #210798

    Purpose

    pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Insert

    MutY (adenine glycosylase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D146N_E45Q

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pkk223_RbMutY_F298A_T299A_H300A

    Plasmid

    #210799

    Purpose

    pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Insert

    MutY (adenine glycosylase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F298A_T299A_H300A

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal Tw

    Plasmid

    #204791

    Purpose

    Bacterial expression of a fusion of Zika NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Zika NS2B-NS3 fusion
    

    Use

    Tags

    6 His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204799

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pRV∆L-5tTA

    Plasmid

    #182966

    Purpose

    The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    tTA
    

    Use

    Rabies

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC-6-ELuc-9

    Plasmid

    #197736

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Emerald/Enhanced Click Beetle Luciferase (ELuc) (tomato codon-optimized)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198049

    Purpose

    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGADT7-αC

    Plasmid

    #197650

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-2 αC
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Ser677T substitution

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H

    Plasmid

    #146493

    Purpose

    Insect Expression of DmeIF4Ga-Nterm

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

    Insert

    DmeIF4Ga-Nterm (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGADT7-AP-1 σ1B

    Plasmid

    #198335

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGADT7-AP-1 σ1A

    Plasmid

    #198334

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENmRFPCNAL2 pc1054

    Plasmid

    #167567

    Purpose

    The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

    Depositor

    Cristina Cardoso

    Article

    Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5/FRT/TO/TARG1

    Plasmid

    #172580

    Purpose

    For generation of FlpIn lines with untagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Mito-CoilE-mEm

    Plasmid

    #181994

    Purpose

    CoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    Mito-CoilE-mEm
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Antares2-N1

    Plasmid

    #120869

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-TK-Firefly-cjCD69

    Plasmid

    #122279

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjCD69 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-TK-Firefly-cjMGA

    Plasmid

    #122280

    Purpose

    Lentiviral vector for stable expression of Firefly luciferase.

    Depositor

    Demián Cazalla

    Article

    Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

    Inserts

    Firefly luciferase
    cjMGA 3'UTR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge